著者

Tomohito Tsukamoto Eiko Sakai Shunsuke Iizuka Marcos Taracena-Gándara Fuminori Sakurai Hiroyuki Mizuguchi

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.41, no.7, pp.1089-1095, 2018-07-01 (Released:2018-07-01)

参考文献数

37

被引用文献数

16

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The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) 9 system is now widely used as a genome editing tool. CRISPR-associated endonuclease in Prevotella and Francisella 1 (Cpf1) is a recently discovered Cas endonuclease that is designable and highly specific with efficiencies comparable to those of Cas9. Here we generated the adenovirus (Ad) vector carrying an Acidaminococcus sp. Cpf1 (AsCpf1) expression cassette (Ad-AsCpf1) for the first time. Ad-AsCpf1 was applied to primary human hepatocytes prepared from humanized mice with chimeric liver in combination with the Ad vector expressing the guide RNA (gRNA) directed to the Adeno-associated virus integration site 1 (AAVS1) region. The mutation rates were estimated by T7 endonuclease I assay around 12% of insertion/deletion (indel). Furthermore, the transduced human hepatocytes were viable (ca. 60%) at two weeks post transduction. These observations suggest that the Ad vector-mediated delivery of the CRISPR/AsCpf1 system provides a useful tool for genome manipulation of human hepatocytes.

著者

酒井 映子 末田 香里 内島 幸江 サカイ エイコ スエダ カオリ ウチジマ ユキエ Eiko SAKAI Kaori SUEDA Yukie UCHIJIMA

雑誌

名古屋女子大学紀要. 家政・自然編 = Journal of Nagoya Women's University. Home economics・natural science

巻号頁・発行日

vol.40, pp.145-153, 1994-03-05

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"中国貴州省吾南部の苗族と布依族の食文化の特徴の一端を栄養的側面から明らかにすることを目的として,日常の食事状況について調査研究を行った.中国における栽培作物の事情は,1980年の「包産到戸」,すなわち農家の個人販売許可によって,食料作物から経済作物へと変化している.このような状況の中で,少数民族である苗族や布依族においても食料事情には変化が生じているものと考えられる.そこで,主として日常の食物摂取状況から両民族間の比較検討を行い,さらに,現状の栄養的問題についても若干の検討を行ったので報告する."