- 著者
- Ayaka Matsuta Takahiro Mayuzumi Hajime Katano Masanori Hatashita Keiichi Takagi Yoriko Hayashi Tomoko Abe Koji Murai Yusuke Kazama
- 出版者
- Japan Mendel Society, International Society of Cytology
- 雑誌
- CYTOLOGIA (ISSN:00114545)
- 巻号頁・発行日
- vol.86, no.4, pp.317-322, 2021-12-25 (Released:2021-12-24)
- 参考文献数
- 43
- 被引用文献数
- 1
A high-LET heavy-ion beam has a severe effect on survival and effectively induces chromosomal rearrangements. In this study, the effect of high-LET heavy-ion irradiation on mutation induction in the M1 generation was investigated in an inbred line of Torenia fournieri, which is a widely used horticultural plant. Dry seeds of the inbred line ‘Zairai murasaki’ were irradiated with a C-ion beam (LET: 50 keV µm−1) or Ar-ion beams (LETs: 184 keV µm−1 or 290 keV µm−1) at different doses, and then sown on 1/2 MS plates. After determining the survival rates from each irradiation condition, appropriate doses of each beam were roughly determined to produce a survival rate of 90%: 300, 75, and 50 Gy for the C-ion beam with a LET of 50 keV µm−1, Ar-ion beam with a LET of 184 keV µm−1, and Ar-ion beam with a LET of 290 keV µm−1, respectively. In the screening of branches with aberrant flowers, one and two aberrant plants were isolated from 16 and 30 M1 plants after irradiation with LETs of 184 keV µm−1 and 290 keV µm−1, respectively. However, no aberrant plants were identified in M1 plants after irradiation with a LET of 50 keV µm−1. We concluded that high-LET heavy-ion beam irradiation is effective in inducing mutations even in the M1 generation of inbred ornamental plants. This technique could be widely used for breeding ornamental plants that can be propagated vegetatively.
- 著者
- Yukina Tatsuta Yukie Tanaka Akari Ikeda Shigeru Matsukawa Hajime Katano Shu Taira
- 出版者
- 日本質量分析学会
- 雑誌
- Mass Spectrometry (ISSN:2187137X)
- 巻号頁・発行日
- vol.6, no.3, pp.S0069-S0069, 2017-09-08 (Released:2017-09-15)
- 参考文献数
- 7
- 被引用文献数
- 8
We compared two ionization methods, matrix assisted laser desorption/ionization (MALDI) and nanoparticle assisted laser desorption/ionization (Nano-PALDI) mass spectrometry (MS), for the analysis of amino acids derivatized with Py-Tag™ that consists pyrylium-based compound. Py-Tag is a useful stable derivatization reagent due to wide mass differences (using 13C as the sole stable labelling isotope). For Py-Tag labelled lysine, sensitive signals that showed less noise with a ten times higher sensitivity, showed a wider mass difference by Nano-PALDI MS compared to MALDI MS.