著者

Xiaoyin SUN Takao YASUI Takeshi YANAGIDA Noritada KAJI Sakon RAHONG Masaki KANAI Kazuki NAGASHIMA Tomoji KAWAI Yoshinobu BABA

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.33, no.6, pp.735-738, 2017-06-10 (Released:2017-06-10)

参考文献数

10

被引用文献数

1

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Here, we developed a device integrated with a nanochannel and nanostructures to slow DNA translocation velocity. We found that translocation velocity of a single DNA molecule inside a nanochannel was decreased by pre-elongating it using some nanostructures, such as a shallow channel or nanopillars. This decrease of the translocation velocity was associated with the DNA mobility change, which is an intrinsic parameter of DNA molecules and unaffected by an electric field.

著者

Kazuki Nagashima Kojiro Hiruma Eri Nakamura Machiko Watanabe Yuko Sekine

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

pp.b23-00530, (Released:2023-11-16)

参考文献数

27

被引用文献数

1

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Overdose has become a global social problem. The Japanese government requires gatekeeper training to detect and prevent indicators of overdose and suicide. However, knowledge of necessary factors for the gatekeeper of overdose (patient intervention) is limited. This study aimed to investigate the characteristics of individuals who experienced intervening persons expected to overdose, and to identify the factors required of gatekeepers. A Google form was used to survey 298 pharmacists and registered sellers in Japan. We searched for factors by logistic analysis. Knowledge of prescription drugs used for overdose was higher among pharmacists than among registered sellers. Conversely, pharmacists and registered sellers had similar knowledge about OTC drugs. Overall multivariate logistic regression analysis revealed countermeasures against overdose at their workplace (odds ratio [OR]: 4.01, 95% confidence interval [CI]: 2.25–7.15, P  <  0.01) and knowledge that overdose is on the rise (OR: 1.93, 95% CI: 1.04–3.69, P  <  0.05) to be significantly associated with intervention experience as a gatekeeper. Countermeasures against overdose at their workplace (OR: 2.40, 95% CI: 1.10–5.25, P  <  0.05) in pharmacists and years of work experience (OR: 1.13, 95% CI: 1.04–1.24, P  <  0.05), countermeasure against overdose at their workplace (OR: 3.43, 95% CI: 1.18–10.0, P  <  0.05), and willingness to participate in study sessions and workshops on overdose (OR: 3.50, 95% CI: 1.51–8.10, P  <  0.05) in registered seller were significantly associated with intervention experience as a gatekeeper. These results are useful evidences for countermeasures and gatekeeper training for overdose at pharmacies and drugstores in the community.

著者

Sakon RAHONG Takao YASUI Takeshi YANAGIDA Kazuki NAGASHIMA Masaki KANAI Gang MENG Yong HE Fuwei ZHUGE Noritada KAJI Tomoji KAWAI Yoshinobu BABA

出版者

(社)日本分析化学会

雑誌

Analytical Sciences (ISSN:09106340)

巻号頁・発行日

vol.31, no.3, pp.153-157, 2015-03-10 (Released:2015-03-10)

参考文献数

23

被引用文献数

3 12

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Molecular filtration and purification play important roles for biomolecule analysis. However, it is still necessary to improve efficiency and reduce the filtration time. Here, we show self-assembled nanowire arrays as three-dimensional (3D) nanopores embedded in a microfluidic channel for ultrafast DNA filtration. The 3D nanopore structure was formed by a vapor-liquid-solid (VLS) nanowire growth technique, which allowed us to control pore size of the filtration material by varying the number of growth cycles. λ DNA molecules (48.5 kbp) were filtrated from a mixture of T4 DNA (166 kbp) at the entrance of the 3D nanopore structure within 1 s under an applied electric field. Moreover, we observed single DNA molecule migration of T4 and λ DNA molecules to clarify the filtration mechanism. The 3D nanopore structure has simplicity of fabrication, flexibility of pore size control and reusability for biomolecule filtration. Consequently it is an excellent material for biomolecular filtration.