著者

川端 健司 Kenji Kawabata

出版者

同志社大学政策学会

雑誌

同志社政策科学研究 = Doshisha University policy & management review (ISSN:18808336)

巻号頁・発行日

vol.22, no.1, pp.79-90, 2020-08-01

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90年代の不登校の増加は、子どもの心理発達の調整局面というより、子育て世帯に広がる生活困窮の広がりが強く作用した結果である。確かに、子どもの貧困率は2015年に改善が見られたものの、むしろ、母子世帯において格差が広がり、所得低位層に援助拒否が見られる。2009年より始まる訪問型家庭教育支援は、地域の人が親との雑談を大切にした支援を行ない、親の自己肯定感の回復につながり、不登校の子どもに就学意欲の改善が見られた。研究ノート(博士資格論文)(Note(Doctoral qualification thesis))

著者

Tomoko Yamaguchi Misae Nishijima Kenji Kawabata

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.45, no.10, pp.1525-1530, 2022-10-01 (Released:2022-10-01)

参考文献数

27

被引用文献数

3

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Brain microvascular endothelial cells (BMECs) are essential component of the blood–brain barrier (BBB). BMECs strictly regulate the entry of various molecules into the central nervous system from the peripheral circulation by forming tight junctions and expressing various influx/efflux transporters and receptors. In vitro BBB models have been widely reported with primary BMECs isolated from animals, although it is known that the expression patterns and levels of transporters and receptors in BMECs differ between humans and animals. Recently, several methods to differentiate BMECs from human induced pluripotent stem (hiPS) cell have been developed. However, the expression of P-glycoprotein (P-gp), which is a key efflux transporter, in hiPS cell-derived BMECs was detected at a relatively low level compared with primary human BMECs. In this study, we examined the involvement of the canonical Wnt signaling pathway, which contributes to the development of BBB formation, in the regulation of P-gp expression in hiPS cell-derived BMECs. We found that the barrier integrity was significantly enhanced in hiPS cell-derived BMECs treated with glycogen synthase kinase-3ß (GSK-3ß) inhibitors, which are known to positively regulate the canonical Wnt signaling pathway. In addition, our data also showed P-gp expression level was increased by treatment with GSK-3ß inhibitors. In conclusion, physiological barrier function and P-gp expression in BMECs can be enhanced by the canonical Wnt signaling pathway. Our results may be useful for promoting the development of drugs for central nervous system diseases using in vitro BBB model.