- 著者
- HIROSHI NISHIOKA TSUTOMU SAWA KUNIO ISSHIKI YOSHIKAZU TAKAHASHI HIROSHI NAGANAWA NAOKO MATSUDA SEIKO HATTORI MASA HAMADA TOMIO TAKEUCHI KAZUO UMEZAWA
- 出版者
- JAPAN ANTIBIOTICS RESEARCH ASSOCIATION
- 雑誌
- The Journal of Antibiotics (ISSN:00218820)
- 巻号頁・発行日
- vol.44, no.11, pp.1283-1285, 1991-11-25 (Released:2006-04-19)
- 参考文献数
- 8
- 被引用文献数
- 6 12
1 0 0 0 OA STUDIES ON A NEW EPIDERMAL GROWTH FACTOR-RECEPTOR KINASE INHIBITOR, ERBSTATIN, PRODUCED BY MH435-hF3
- 著者
- HAMAO UMEZAWA MASAYA IMOTO TSUTOMU SAWA KUNIO ISSHIKI NAOKO MATSUDA TAKESHI UCHIDA HIRONOBU IINUMA MASA HAMADA TOMIO TAKEUCHI
- 出版者
- JAPAN ANTIBIOTICS RESEARCH ASSOCIATION
- 雑誌
- The Journal of Antibiotics (ISSN:00218820)
- 巻号頁・発行日
- vol.39, no.1, pp.170-173, 1986 (Released:2006-04-19)
- 参考文献数
- 15
- 被引用文献数
- 123 159
- 著者
- KAYOKO SUZUKAKE-TSUCHIYA MAKOTO HORI NOBUYOSHI SHIMADA MASA HAMADA
- 出版者
- JAPAN ANTIBIOTICS RESEARCH ASSOCIATION
- 雑誌
- The Journal of Antibiotics (ISSN:00218820)
- 巻号頁・発行日
- vol.41, no.5, pp.675-683, 1988-05-25 (Released:2006-04-19)
- 参考文献数
- 6
- 被引用文献数
- 3 5
Deoxypheganomycin D, a specific inhibitor of mycobacteria, inhibits the growth in vitro of Mycobacterium smegmatis ATCC 607 (M. 607) bacteriostatically at concentrations as high as 7×10-5M. It shows no cross-resistance to paromomycin, capreomycin, viomycin, streptothricin, kanamycin and streptomycin. Deoxypheganomycin D at 2.8×10-7M where the cell growth of M. 607 is only partially inhibited does not significantly inhibit DNA, RNA or protein synthesis but leads to marked decrease (13 % of control) in [14C]glycerol-derived radioactivity in cell-walls. In the presence of 7×10-6M deoxypheganomycin D, the influx of leucine but not thymidine is affected while the reverse is true with efflux. The data suggest that the effect of deoxypheganomycin D on M. 607 may be related to both the cell membrane and specific mycobacterial lipid like components of the cell-wall.
- 著者
- Naofumi Tsuchizaki Masa Hamada Kunimoto Hotta
- 出版者
- 日本放線菌学会
- 雑誌
- 日本放線菌学会誌 (ISSN:09145818)
- 巻号頁・発行日
- vol.15, no.2, pp.23-29, 2001-12-25 (Released:2008-03-04)
- 参考文献数
- 26
- 被引用文献数
- 2 3
(Using colony direct PCR we established, kan gene (GenBank accession No. AB028210) coding for an aminoglycoside acetyltransferase, AAC(3), was surveyed among strains of Streptomyces griseus and its related taxa (S. anulatus group). The regulatory and ORF regions of kan gene were targeted by the use of original primers. With the regulatory region primers, the target fragment (518 bp) amplification was observed in all the streptomycin (SM)-producing strains of S. griseus and in several strains of S. anulatus group. The other target fragment (930 bp) amplification using the ORF primers was observed only in the SM-producing strains of S. griseus including ‘S. ornatus’ that has substantially identical properties to those of S. griseus. It was thus conclusive that kan gene is highly conserved in SM-producing strains of S. griseus and distributed in taxa related to S. griseus. There were numbers of strains that provided no amplification upon PCR but hybridization bands upon Southern hybridization, suggesting the lack or alteration of kan gene. Characterization by colony direct PCR using appropriate primers will be a big help for taxonomic as well as ecological characterization of actinomycetes.