著者

Manabu Onuma

出版者

Japan Poultry Science Association

雑誌

The Journal of Poultry Science (ISSN:13467395)

巻号頁・発行日

pp.0150015, (Released:2015-04-25)

被引用文献数

1

---

As of 2014, 89 species have been legally designated as “nationally endangered species of wild fauna and flora,” including 37 avian species in Japan. To facilitate the breeding of endangered species and the maintenance of protected habitats, a program for the rehabilitation of natural habitats and maintenance of viable populations is being established by the Ministry of the Environment and other related ministries for 49 of the species on the above list. The programs have already been established for 15 such avian species, and are currently managed by zoos or local authorities. Maintaining genetic diversity is necessary to ensure the stable procreation of endangered species. A decline in breeding potential due to inbreeding can be prevented by careful maintenance of genetic diversity. In addition to the prevention of inbreeding depression, the maintenance of genetic diversity safeguards species’ resistance to pathogens, as well as their capacity to cope with environmental change. This manuscript reports on the status of currently available genetic information for nationally endangered avian species in Japan, with a particular focus on the 15 species included in the conservation and propagation program. In the NCBI “Nucleotide” database, 315 entries were related to sequence information from the 15 endangered species. Mitochondrial DNA-related sequences constituted 238 (75.6%) of these entries. In the NCBI “Genome” database, genome sequence entries for three species were found. As is the case with mitochondrial DNA, microsatellite loci are very useful for endangered species conservation to evaluate genetic diversity. However, information about the endangered avian species’ microsatellite makers is limited. This situation could have a considerable negative impact on captive breeding projects in particular. Hence, the development of microsatellite markers for these species is a priority.

著者

Masafumi KATAYAMA Takashi HIRAYAMA Tohru KIYONO Manabu ONUMA Tetsuya TANI Satoru TAKEDA Katsuhiko NISHIMORI Tomokazu FUKUDA

出版者

日本繁殖生物学会

雑誌

Journal of Reproduction and Development (ISSN:09168818)

巻号頁・発行日

pp.2016-164, (Released:2017-03-23)

被引用文献数

13

---

The cellular conditions required to establish induced pluripotent stem cells (iPSCs), such as the number of reprogramming factors and/or promoter selection, differ among species. The establishment of iPSCs derived from cells of previously unstudied species therefore requires the extensive optimization of programming conditions, including promoter selection and the optimal number of reprogramming factors, through a trial-and-error approach. While the four Yamanaka factors Oct3/4, Sox2, Klf4, and c-Myc are sufficient for iPSC establishment in mice, we reported previously that six reprogramming factors were necessary for the creation of iPSCs from primary prairie vole-derived cells. Further to this study, we now show detailed data describing the optimization protocol we developed in order to obtain iPSCs from immortalized prairie vole-derived fibroblasts. Immortalized cells can be very useful tools in the optimization of cellular reprogramming conditions, as cellular senescence is known to dramatically decrease the efficiency of iPSC establishment. The immortalized prairie vole cells used in this optimization were designated K4DT cells as they contained mutant forms of CDK4, cyclin D, and telomerase reverse transcriptase (TERT). We show that iPSCs derived from these immortalized cells exhibit the transcriptional silencing of exogenous reprogramming factors while maintaining pluripotent cell morphology. There were no observed differences between the iPSCs derived from primary and immortalized prairie vole fibroblasts. Our data suggest that cells that are immortalized with mutant CDK4, cyclin D, and TERT provide a useful tool for the determination of the optimal conditions for iPSC establishment.

著者

Alice CC LAU Yongjin QIU Mohamed Abdallah Mohamed MOUSTAFA Ryo NAKAO Michito SHIMOZURU Manabu ONUMA Nobuyoshi NAKAJIMA Mariko SASHIKA Jayasilan MOHD-AZLAN Toshio TSUBOTA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.84, no.11, pp.1469-1473, 2022 (Released:2022-11-01)

参考文献数

40

---

In this study, Babesia screening was conducted in 55 rodents and 160 tick samples collected from primary forests and an oil palm plantation in Sarawak, Malaysian Borneo. PCR targeting the 18S ribosomal DNA revealed the presence of Babesia spp. DNA detected in two questing male Haemaphysalis shimoga ticks collected from the oil palm plantation. Sequence analysis revealed that both sequences were identical and had 98.6% identity to a Babesia macropus sequence obtained from Eastern grey kangaroos (Macropus giganteus) in Australia. Phylogenetic tree revealed clustering with marsupial-associated Babesia spp. in the Babesia sensu stricto clade. Whether or not H. shimoga is the competent vector and the importance of the Babesia sp. detected in this study warrants more investigation.

著者

Chihiro ISHII Yoshinori IKENAKA Shouta M.M. NAKAYAMA Takeshi KURITANI Mitsuhiro NAKAGAWA Keisuke SAITO Yukiko WATANABE Kohei OGASAWARA Manabu ONUMA Atsushi HAGA Mayumi ISHIZUKA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.82, no.8, pp.1118-1123, 2020 (Released:2020-08-19)

参考文献数

31

被引用文献数

4

---

Birds of a number of species have died as a result of lead (Pb) poisoning, including many Steller’s sea eagles (Haliaeetus pelagicus) and white-tailed sea eagles (Haliaeetus albicilla) in Hokkaido, the northernmost island of Japan. To address this issue, the use of any type of Pb ammunition for hunting of large animals was prohibited in Hokkaido in 2004. However, Pb poisoning is still being reported in this area, and there are few regulations regarding the use of Pb ammunition in other parts of Japan, where it has been reported that eagles and water birds have been exposed to Pb. This study was performed to accurately determine the current level of Pb exposure of birds found dead in the field or dead in the wild bird centers in Japan (June 2015–May 2018) and to identify the sources of Pb. Pb exposure was found to still be occurring in raptors and water birds in various parts of Japan. Twenty-six point five % and 5.9% of the recorded deaths of Steller’s sea eagles and white-tailed sea eagles, respectively, were found to have been poisoned by Pb. In addition, Pb isotope ratio analysis showed that both Pb rifle bullets and Pb shot pellets cause Pb exposure in birds, and these endangered eagles are also exposed to Pb in Hokkaido due to the illegal use of Pb ammunition. Changing to Pb-free ammunition, such as copper (Cu) rifle bullets, steel shot pellets, or bismuth shot pellets, will be essential for the conservation of avian species in Japan.

著者

Katsuro HAGIWARA Takaaki NAKAYA Manabu ONUMA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

pp.19-0408, (Released:2020-03-16)

被引用文献数

1

---

We compared the Mx expression and anti-viral function and the 3D structure of Mx protein in four species: chicken (Gallus gallus), whooper swan (Cygnus cygnus), jungle crow (Corvus macrorhynchos), and rock dove (Columba livia). We observed different mortalities associated with highly pathogenic avian influenza virus (HPAIV) infection to understand the relationship between Mx function as an immune response factor and HPAIV proliferation in bird cells. Different levels of Mx were observed among the different bird species after virus infection. Strong Mx expression was confirmed in the rock dove and whooper swan 6 hr after viral infection. The lowest virus copy numbers were observed in rock dove. The virus infectivity was significantly reduced in the BALA/3T3 cells expressing rock dove and jungle crow Mx. These results suggested that high Mx expression and significant Mx-induced anti-viral effects might result in the rock dove primary cells having the lowest virus copy number. Comparison of the expected 3D structure of Mx protein in all four bird species demonstrated that the structure of loop L4 varied among the investigated species. It was reported that differences in amino acid sequence in loop L4 affect antiviral activity in human and mouse cells, and a significant anti-viral effect was observed in the rock dove Mx. Thus, the amino acid sequence of loop L4 in rock dove might represent relatively high anti-viral activity.

著者

Manabu ONUMA Masayoshi KAKOGAWA Masae YANAGISAWA Atsushi HAGA Tomomi OKANO Yasuko NEAGARI Tsukasa OKANO Koichi GOKA Mitsuhiko ASAKAWA

出版者

公益社団法人 日本獣医学会

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.79, no.5, pp.943-951, 2017 (Released:2017-05-23)

参考文献数

39

被引用文献数

17

---

The objectives of the present study were to observe the temporal pattern of avian influenza virus (AIV) introduction into Japan and to determine which migratory birds play an important role in introducing AIV. In total, 19,407 fecal samples from migratory birds were collected at 52 sites between October 2008 and May 2015. Total nucleic acids extracted from the fecal samples were subjected to reverse transcription loop–mediated isothermal amplification to detect viral RNA. Species identification of host migratory birds was conducted by DNA barcoding for positive fecal samples. The total number of positive samples was 352 (prevalence, 1.8%). The highest prevalence was observed in autumn migration, and a decrease in prevalence was observed. During autumn migration, central to southern Japan showed a prevalence higher than the overall prevalence. Thus, the main AIV entry routes may involve crossing the Sea of Japan and entry through the Korean Peninsula. Species identification was successful in 221 of the 352 positive samples. Two major species sequences were identified: the Mallard/Eastern Spot-billed duck group (115 samples; 52.0%) and the Northern pintail (61 samples; 27.6%). To gain a better understanding of the ecology of AIV in Japan and the introduction pattern of highly pathogenic avian influenza viruses, information regarding AIV prevalence by species, the prevalence of hatch-year migratory birds, migration patterns and viral subtypes in fecal samples using egg inoculation and molecular-based methods in combination is required.