著者

中村 雅典 Masanori Nakamura

出版者

東北大学歯学会

雑誌

東北大学歯学雑誌 = Tohoku University dental journal (ISSN:02873915)

巻号頁・発行日

vol.21, no.1, pp.9-17, 2002-06-01

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破骨細胞が骨破壊の主たる細胞であることはいうまでもないことであるが, 他の細胞による骨破壊が完全に否定されているわけではない。ビスフォスフォネート(BP)は破骨細胞による骨吸収・破壊を特異的に抑制する薬剤である。そこで, 我々はBPを投与したコラーゲン誘導関節炎(CIA)マウス, 並びに重症慢性リウマチ性関節炎(RA)患者の骨破壊機構についてFlow Cytometryと形態学的に解析を行った。CIAマウスでは, BP投与・非投与に関わらず著しい骨破壊が認められた。非投与群における骨破壊部位には活性化した破骨細胞はなく, 多くの好中球が集積し, 一部はruptureし, この部位の骨基質からコラーゲン線維が消失していた。RA患者腸骨骨髄では, 著しい顆粒球造血, 特に未熟好中球の増加が認められた。この未熟好中球は骨梁表面に集積しており, CIA同様に一部はruptureし, 骨基質からコラーゲン線維が消失していた。以上の結果から, 関節炎の骨破壊時には好中球造血の異常な亢進が起こり, この好中球による骨破壊が強く示唆される。近年, また, 関節炎だけでなく, 歯周疾患のような骨破壊を主体とする他の疾患(歯周疾患など)においても同様な造血異常が報告されてきており, 造血という全身に立脚した骨破壊性疾患の解析が期待される。Osteoclasts are the main cells responsible for bone destruction and resorption However, whether cells other than osteoclasts destruct bone remains controversial.Bisphosphonates(BPs)are specific inhibitors of bone resorption by osteoclasts.We examined the effects of BPs on bone destruction in mice with collagen-induoed arthritis(CIA).Severe bone destruction was confirmed in BP-treated CIA mice, indicating bone destruction by cells other than osteoclasts.Histological and flow cytometrical studies showed Increased granulopoiesis and bone destruction by neutrophils.Similar results were obtained in studies of patients with severe rheumatoid arthritis. In this paper, we describe the process of and data derived from our experimental strategy and review possible mechanisms of bone destruction by neutrophils.

著者

Shinji Goto Masanori Nakamura Keiichi Itatani Shohei Miyazaki Norihiko Oka Takashi Honda Tadashi Kitamura Tetsuya Horai Masahiro Ishii Kagami Miyaji

出版者

International Heart Journal Association

雑誌

International Heart Journal (ISSN:13492365)

巻号頁・発行日

pp.15-440, (Released:2016-07-07)

被引用文献数

4 6

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The use of measured data as boundary conditions renders hemodynamic simulations more patient-specific. However, synchronized acquisition of data at multiple locations is often difficult in clinical practice. This study proposes a method for resynchronizing measured data for use as boundary conditions for flow simulations using frequency analyses, and discusses the optimal cut-off frequency for differentiating cardiac and respiratory variation in hemodynamic data during resynchronization. To demonstrate the utility of the method, a Fontan circulation, which is the final palliative result with single-ventricle physiology, was used. The results suggest that it is optimal to set a cut-off frequency that gives a local minimum in the power spectrum that is slightly lower than the peak frequency of the heartbeat. Additionally, the total energy loss depended on the cut-off frequency, although the overall flow patterns appeared to be similar. The method is applicable to cardiovascular systems other than the Fontan circulation, where hemodynamic data with multifactorial fluctuations are required at various locations but simultaneous measurements are not possible.