- 著者
- Toshikazu TAMURA Nobutaka WATANABE Aki YAMAMURA Mizuyo OURA Yukie TAKATSUKI Tetsuya HISATAKE Satoshi IMAZATO Tositomo KANAKUBO Satoko TANIYA Kuniaki USHIGOE Hiromi TANAKA Ryota NAGASAWA Rie WATANABE Shigeru KOBAYASHI
- 出版者
- The Association of Japanese Geographers
- 雑誌
- 地理学評論 (ISSN:13479555)
- 巻号頁・発行日
- vol.78, no.5, pp.332-337, 2005-04-01 (Released:2008-12-25)
- 著者
- Maho URATA Rie WATANABE Hiroyuki IWATA
- 出版者
- 公益社団法人 日本獣医学会
- 雑誌
- Journal of Veterinary Medical Science (ISSN:09167250)
- 巻号頁・発行日
- vol.77, no.12, pp.1611-1616, 2015 (Released:2016-01-01)
- 参考文献数
- 28
- 被引用文献数
- 2
The cytotoxicity of Ibaraki virus nonstructural protein NS3 was confirmed, and the contribution of glycosylation to this activity was examined by using glycosylation mutants of NS3 generated by site-directed mutagenesis. The expression of NS3 resulted in leakage of lactate dehydrogenase to the culture supernatant, suggesting the cytotoxicity of this protein. The lack of glycosylation impaired the transport of NS3 to the plasma membrane and resulted in reduced cytotoxicity. Combined with the previous observation that NS3 glycosylation was specifically observed in mammalian cells (Urata et al., Virus Research 2014), it was suggested that the alteration of NS3 cytotoxicity through modulating glycosylation is one of the strategies to achieve host specific pathogenisity of Ibaraki virus between mammals and vector arthropods.