著者

Riho Komatsuzawa Teruo Miyazaki Hajime Ohmori Chitose Maruyama Stephen W. Schaffer Shigeru Murakami Takashi Ito

出版者

The Japanese Society of Physical Fitness and Sports Medicine

雑誌

The Journal of Physical Fitness and Sports Medicine (ISSN:21868131)

巻号頁・発行日

vol.9, no.4, pp.165-171, 2020-07-25 (Released:2020-07-15)

参考文献数

28

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The amount of taurine and taurine derivatives in the body is affected by various diseases and physiological events, such as exercise. However, there is little information about possible changes in taurine distribution within tissue. In the present study, we examined whether matrix-assisted laser desorption/ionization-imaging mass spectrometry (MALDI-IMS) can be used to determine the effect of exercise on the distribution and content of taurine and acetyltaurine, a taurine derivative present in skeletal muscle. Using 9-aminoacridine to detect the amino acids in tissue samples, the content of taurine and acetyltaurine in homogenates of skeletal muscle was measured by MALDI-IMS. While the intrinsic levels of taurine in skeletal muscle tissue were adequate to be detected by MALDI-IMS, that of acetyltaurine was not observed. Following 120-min of treadmill running (20m/min), taurine content of soleus and plantaris muscles significantly declined. In the gastrocnemius muscle, taurine content is higher in slow-twitch fiber than fast-twitch fiber. However, the taurine content was not significantly changed by treadmill running in both fast- and slow-twitch fiber of the gastrocnemius muscle. In conclusion, MALDI-IMS using 9-aminoacridine as a matrix could detect the distribution of taurine in skeletal muscle before and after exercise.

著者

Takashi Ito Yuuki Hanahata Keita Kine Shigeru Murakami Stephen W. Schaffer

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.41, no.10, pp.1561-1566, 2018-10-01 (Released:2018-10-01)

参考文献数

29

被引用文献数

9 12

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Dietary taurine deficiency results in dilated cardiomyopathy in cats while in mice taurine deficiency produced by knocking out the taurine transporter (TauT) gene leads to a reduction in cardiac function with advancing age. The present study elucidated the involvement of cardiac fibrosis in the aging-dependent cardiac disorder of the TauT-knockout (TauTKO) mouse. Old (18–24-month-old) TauTKO mice, but not young (3–5-month-old) mice, exhibit cardiac fibrosis. Transcriptome microarray analysis revealed an increase in pro-fibrotic genes, such as S100A4, ACTA2 and CTGF, in both young and old TauTKO hearts. Based on transcriptome-pathway analysis the genes involved in “organization of extracellular matrix,” such as LGALS3, are enriched in old TauTKO hearts compared to old wild-type hearts, suggesting the contribution of these genes to fibrosis. In conclusion, taurine depletion predisposes the heart to fibrosis, which leads to cardiac fibrosis upon aging.