- 著者
- Ken Kuroki Kai Yan Hiroyoshi Iwata Kentaro K. Shimizu Toshiaki Tameshige Shuhei Nasuda Wei Guo
- 出版者
- Japanese Society of Breeding
- 雑誌
- Breeding Science (ISSN:13447610)
- 巻号頁・発行日
- vol.72, no.1, pp.66-74, 2022 (Released:2022-03-08)
- 参考文献数
- 28
Phenotyping is a critical process in plant breeding, especially when there is an increasing demand for streamlining a selection process in a breeding program. Since manual phenotyping has limited efficiency, high-throughput phenotyping methods are recently popularized owing to progress in sensor and image processing technologies. However, in a size-limited breeding field, which is common in Japan and other Asian countries, it is challenging to introduce large machinery in the field or fly unmanned aerial vehicles over the field. In this study, we developed a ground-based high-throughput field phenotyping rover that could be easily introduced to a field regardless of the scale and location of the field even without special facilities. We also made the field rover open-source hardware, making its system available to public for easy modification, so that anyone can build one for their own use at a low cost. The trial run of the field rover revealed that it allowed the collection of detailed remote-sensing images of plants and quantitative analyses based on the images. The results suggest that the field rover developed in this study could allow efficient phenotyping of plants especially in a small breeding field.
- 著者
- Shuhei Nasuda Kazuki Murata Hiroyuki Kakui Koichi Yamamori Takanori Yoshikawa
- 出版者
- Japan Mendel Society, International Society of Cytology
- 雑誌
- CYTOLOGIA (ISSN:00114545)
- 巻号頁・発行日
- vol.88, no.3, pp.167-168, 2023-09-25 (Released:2023-09-25)
- 参考文献数
- 4
- 著者
- Naoki Shitsukawa Chihiro Ikari Sanae Shimada Satoshi Kitagawa Koichi Sakamoto Hiroyuki Saito Hiromichi Ryuto Nobuhisa Fukunishi Tomoko Abe Shigeo Takumi Shuhei Nasuda Koji Murai
- 出版者
- The Genetics Society of Japan
- 雑誌
- Genes & Genetic Systems (ISSN:13417568)
- 巻号頁・発行日
- vol.82, no.2, pp.167-170, 2007 (Released:2007-05-11)
- 参考文献数
- 13
- 被引用文献数
- 88 98
The einkorn wheat (Triticum monococcum) mutant, maintained vegetative phase (mvp), was induced by nitrogen ion-beam treatment and was identified by its inability to transit from the vegetative to reproductive phase. In our previous study, we showed that WAP1 (wheat APETALA1) is a key gene in the regulatory pathway that controls phase transition from vegetative to reproductive growth in common wheat. WAP1 is an ortholog of the VRN1 gene that is responsible for vernalization insensitivity in einkorn wheat. The mvp mutation resulted from deletion of the VRN1 coding and promoter regions, demonstrating that WAP1/VRN1 is an indispensable gene for phase transition in wheat. Expression analysis of flowering-related genes in mvp plants indicated that wheat GIGANTIA (GI), CONSTANS (CO) and SUPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) genes either act upstream of or in a different pathway to WAP1/VRN1.
- 著者
- Masashi Tsuchida Takehiro Fukushima Shuhei Nasuda Ali Masoudi-Nejad Goro Ishikawa Toshiki Nakamura Takashi R. Endo
- 出版者
- The Genetics Society of Japan
- 雑誌
- Genes & Genetic Systems (ISSN:13417568)
- 巻号頁・発行日
- vol.83, no.1, pp.43-53, 2008 (Released:2008-03-28)
- 参考文献数
- 41
- 被引用文献数
- 22 28
Rye chromosome 1R contains many agronomically useful genes. Physical dissection of chromosome 1R into segments would be useful in mapping 1R-specific DNA markers and in assembling DNA clones into contig maps. We applied the gametocidal system to produce rearranged 1R chromosomes of Imperial rye (1Ri) added to common wheat. We identified rearranged 1Ri chromosomes and established 55 1Ri dissection lines of common wheat carrying a single rearranged 1Ri chromosome. Fifty-two of the rearranged 1Ri chromosomes had single breakpoints and three had double breakpoints. The 58 breakpoints were distributed in the short arm excluding the satellite (12 breakpoints), in the satellite (4), in the long arm (28), and in the centromere (14). Out of the 55 lines, nine were homozygous for the rearranged 1Ri chromosomes, and the remaining lines were hemizygous. We developed 26 PCR-based EST markers that were specific to the 1Ri chromosome, and nine of them amplified 1Ri arm-specific PCR products without restriction-enzyme digestion. Using the nine EST markers and two previously reported 1R-specific markers, we characterized the 55 1Ri dissection lines, and also proved that we can select critical progeny plants carrying specific rearranged 1Ri chromosomes by PCR, without cytological screening, in 48 out of the 55 hemizygous dissection lines.