著者

Ken Kuroki Kai Yan Hiroyoshi Iwata Kentaro K. Shimizu Toshiaki Tameshige Shuhei Nasuda Wei Guo

出版者

Japanese Society of Breeding

雑誌

Breeding Science (ISSN:13447610)

巻号頁・発行日

vol.72, no.1, pp.66-74, 2022 (Released:2022-03-08)

参考文献数

28

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Phenotyping is a critical process in plant breeding, especially when there is an increasing demand for streamlining a selection process in a breeding program. Since manual phenotyping has limited efficiency, high-throughput phenotyping methods are recently popularized owing to progress in sensor and image processing technologies. However, in a size-limited breeding field, which is common in Japan and other Asian countries, it is challenging to introduce large machinery in the field or fly unmanned aerial vehicles over the field. In this study, we developed a ground-based high-throughput field phenotyping rover that could be easily introduced to a field regardless of the scale and location of the field even without special facilities. We also made the field rover open-source hardware, making its system available to public for easy modification, so that anyone can build one for their own use at a low cost. The trial run of the field rover revealed that it allowed the collection of detailed remote-sensing images of plants and quantitative analyses based on the images. The results suggest that the field rover developed in this study could allow efficient phenotyping of plants especially in a small breeding field.

著者

Hiroyoshi Iwata Makoto Kaneko Takao Wakabayashi Shinichiro Ueda

出版者

The Japanese Society of Internal Medicine

雑誌

Internal Medicine (ISSN:09182918)

巻号頁・発行日

pp.9338-22, (Released:2022-02-26)

参考文献数

5

被引用文献数

1

著者

Kazutoshi Yashiro Hiroyoshi Iwata Yukari Akashi Ken-o Tomita Maki Kuzuya Yoshihiko Tsumura Kenji Kato

出版者

Japanese Society of Breeding

雑誌

Breeding Science (ISSN:13447610)

巻号頁・発行日

vol.55, no.2, pp.197-206, 2005 (Released:2005-06-15)

参考文献数

38

被引用文献数

14 27

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Ninety-nine accessions of melon (Cucumis melo L.) mainly from East and South Asia were analyzed based on the polymorphism of 210 amplified fragment length polymorphism (AFLP) bands to reveal the genetic structure and phylogenetic relationship in Asian melon. A cluster analysis based on their genetic similarity revealed three major clusters, i.e., a vars. makuwa and conomon group, a small-seed type group and a group of Japanese F1 cultivars and large-seed type accessions. Most of the East Asian melon accessions classified into the first group were of the small-seed type with a seed length shorter than 9.0 mm. The varieties of C. melo were roughly divided into two groups by a principal co-ordinate analysis based on AFLP data, that is, the group of vars. makuwa and conomon and small-seed type melon and the group of var. reticulatus and large-seed type melon. Indian melon accessions were rich in genetic variation. Melon accessions closely related to vars. makuwa and conomon were found in east India, and they were considered as possible candidates of the prototype of vars. makuwa and conomon.

著者

Shigeki Moriya Miyuki Kunihisa Kazuma Okada Hiroshi Iwanami Hiroyoshi Iwata Mai Minamikawa Yuichi Katayose Toshimi Matsumoto Satomi Mori Harumi Sasaki Takashi Matsumoto Chikako Nishitani Shingo Terakami Toshiya Yamamoto Kazuyuki Abe

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-156, (Released:2016-07-23)

被引用文献数

21

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During apple (Malus × domestica Borkh.) storage, a loss in fruit firmness can occur. This is frequently associated with mealiness, an undesirable trait. There have been studies, such as phenotypic analyses and transcriptomics, as well as others employing a transgenic approach, focusing on this trait. Certain genetic approaches, such as quantitative trait loci (QTL) approach, however, have not been attempted. In this study, to identify and characterize QTLs controlling flesh mealiness and to investigate their application in apple breeding, we performed classical QTL mapping based on a bi-parental population and a genome-wide association study (GWAS) of mealiness. Phenotypic data for mealiness allowed us to identify two QTLs in the bi-parental family located on linkage group 10. The GWAS discovered significant marker-trait associations on chromosomes 2, 9, and 10. The MdPG1 locus, located on chromosome 10, was identified as the locus with the strongest significance by both QTL mapping and GWAS, suggesting its primary contribution to flesh mealiness. Using a tri-allelic simple sequence repeat marker, Md-PG1SSR10kd, 10 kb downstream of the MdPG1 coding sequence, we divided apple accessions into six groups based on their genotypes. Among the six groups, the Md-PG1SSR10kd genotype “2/2” had the least mealy phenotype.