著者

YUKIMARU SUGIYAMA HIROYUKI KURITA TAKESHI MATSUI SATOSHI KIMOTO TADATOSHI SHIMOMURA

出版者

The Anthropological Society of Nippon

雑誌

Anthropological Science (ISSN:09187960)

巻号頁・発行日

vol.117, no.2, pp.113-119, 2009 (Released:2009-07-24)

参考文献数

31

被引用文献数

22 71

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The quantitative and demographic features of infant-corpse-carrying behavior in Japanese macaques (Macaca fuscata) at Takasakiyama, southern Japan, have been studied over 24 years. More than 91% of the dead infants that were carried by their mothers were abandoned within a week. Mothers of all age classes exhibited this behavior and neither the carrying rate (number of carriers/number of deaths) nor the duration were significantly different between young and older mothers. The sex of the infant was not a decisive factor. Nearly 80% of all cases observed involved infants that had died within 30 days of birth. The oldest infant whose corpse was observed being carried had died at 253 days. The overall carrying rate was 15% when death had occurred within 253 days and 28.7% for infants that died within 30 days of birth. Most mothers whose infants had lived for more than a month abandoned the corpse soon after death. Some females persist in exhibiting behaviors performed towards live infants but the exact reasons for this are unclear at present.

著者

Takeshi Matsui Eiji Takita Seika Oiwa Asuka Yokoyama Ko Kato Kazutoshi Sawada

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

pp.21.0414a, (Released:2021-06-12)

参考文献数

47

被引用文献数

2

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Plant-made oral vaccines can be a cost-effective method to control infectious diseases of humans and farm animals. Pig edema is a bacterial disease caused by enterohemorrhagic Escherichia coli producing the toxin Shiga toxin 2e (Stx2e). In our previous report, we chose the non-toxic B subunit of Stx2e (Stx2eB) as a vaccine antigen, and Stx2eB was expressed in lettuce (Lactuca sativa L., cv. Green wave). We found that a double repeated Stx2eB (2×Stx2eB) accumulates to higher levels than a single Stx2eB. In this study, we analyzed progeny plants introduced with 2×Stx2eB in which the gene was expressed under the control of conventional cauliflower mosaic virus 35S RNA (CaMV 35S) promoter, and found that the lettuce underwent transgene silencing and bore few seeds. We resolved these problems by using a transgene cassette which harbored a transcriptional promoter derived from the lettuce ubiquitin gene and a longer version of HSPT. The lettuce harboring this expression construct will be valuable in establishing the seed lot system on the basis that thousands of seeds can be obtained from one plant body and the resulting progeny plants accumulate 2×Stx2eB at high levels without the transgene silencing.

著者

Takeshi Matsui Eiji Takita Seika Oiwa Asuka Yokoyama Ko Kato Kazutoshi Sawada

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.38, no.2, pp.239-246, 2021-06-25 (Released:2021-06-25)

参考文献数

47

被引用文献数

2

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Plant-made oral vaccines can be a cost-effective method to control infectious diseases of humans and farm animals. Pig edema is a bacterial disease caused by enterohemorrhagic Escherichia coli producing the toxin Shiga toxin 2e (Stx2e). In our previous report, we chose the non-toxic B subunit of Stx2e (Stx2eB) as a vaccine antigen, and Stx2eB was expressed in lettuce (Lactuca sativa L., cv. Green wave). We found that a double repeated Stx2eB (2×Stx2eB) accumulates to higher levels than a single Stx2eB. In this study, we analyzed progeny plants introduced with 2×Stx2eB in which the gene was expressed under the control of conventional cauliflower mosaic virus 35S RNA (CaMV 35S) promoter, and found that the lettuce underwent transgene silencing and bore few seeds. We resolved these problems by using a transgene cassette which harbored a transcriptional promoter derived from the lettuce ubiquitin gene and a longer version of HSPT. The lettuce harboring this expression construct will be valuable in establishing the seed lot system on the basis that thousands of seeds can be obtained from one plant body and the resulting progeny plants accumulate 2×Stx2eB at high levels without the transgene silencing.