著者

Taiga YAMAZAKI Satoru KOBAYAKAWA Kazuo YAMAGATA Kuniya ABE Tadashi BABA

出版者

The Society for Reproduction and Development

雑誌

Journal of Reproduction and Development (ISSN:09168818)

巻号頁・発行日

vol.53, no.5, pp.1035-1041, 2007 (Released:2007-10-31)

参考文献数

24

被引用文献数

11 13

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To elucidate the molecular dynamics of HP1β in mouse preimplantation embryos, we examined the localization, dynamics, and mobility of HP1β in the (pro)nucleus by live cell imaging. Time-lapse observation revealed that the chromatin association of HP1β is regulated in a cell cycle-dependent manner. HP1β was localized in the interphase nucleus and was dynamically dissociated from the nucleus during the metaphase stage. The HP1β assembly and clustered heterochromatin structure were both found in the nuclei of 2-cell and later-stage embryos. Moreover, fluorescent recovery after photobleaching analysis implied that HP1β is more freely mobile in the pronucleus of the 1-cell embryo than in the 4-cell nucleus. These results suggest that the chromatin configuration may be regulated by the stability and mobility of chromatin-associated proteins including HP1β during early embryonic stages.

著者

Yoshitaka Kawai Tadashi Baba Mika Yoshida Josephine U. Agravante Dormita R. Del Carmen

出版者

The Japanese Society for Horticultural Science

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.OKD-145, (Released:2018-02-08)

被引用文献数

3

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In harvested calamondin fruit, the effects of benzyladenine (BA) and light on the rind color and fruit quality were investigated. BA application delayed degreening of the calamondin fruit in both light and dark conditions. At 5 days, BA application had no influence on total soluble solids (TSS), titratable acid (TA), sugar contents, ascorbic acid (AA), and organic acid in the fruit juice and at 9 days, only AA content has decreased in BA-treated fruits. Light promoted quick degreening of calamondin fruit, whereas in the dark, degreening had proceeded very little in un-treated fruit at 9 days. Light did not influence fruit quality at 5 days either. However, light influenced the sugar content, especially increasing glucose and total sugar, as well as AA content in fruit at 9 days. Concerning the AA content in calamondin fruit, BA decreased and light increased it. These results indicated that BA treatment after harvest and maintaining fruit in the dark are sufficient to retain the green rind color of harvested calamondin fruit without affecting fruit quality, except for AA content.

著者

Misuzu YAMASHITA Kazuo YAMAGATA Keiko TSUMURA Tomoko NAKANISHI Tadashi BABA

出版者

日本繁殖生物学会

雑誌

Journal of Reproduction and Development (ISSN:09168818)

巻号頁・発行日

vol.53, no.2, pp.255-262, 2007 (Released:2007-05-12)

参考文献数

28

被引用文献数

9 15 14

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To improve assessment of the acrosome reaction of mouse epididymal sperm, we employed anti-Izumo1 antibody instead of antibodies against acrosomal proteins. The acrosomal states among acrosome-intact, spontaneously acrosome-reacted, truly acrosome-reacted, and probably dead and/or membrane-damaged sperm were clearly distinguished by combined application of anti-Izumo1 antibody, DNA dye Hoechst 33342, and monoclonal antibody MN7 to paraformaldehyde-fixed sperm. When the acrosome reaction of capacitated epididymal sperm on the oocyte zona pellucida was examined using anti-Izumo1 antibody, approximately 20% of sperm bound onto the zona pellucida were acrosome-reacted 30 min after insemination. We also observed the moment of the acrosome reaction of live sperm on the zona pellucida by time-lapse monitoring using fluorescein isothiocyanate-conjugated anti-Izumo1 antibody.