著者

Kimura Masahiro Shindo Mitsuno Moriizumi Toshiyuki Tagawa Noriko Fujinami Aya Kato Ikuo Uchida Yoshiki

出版者

公益社団法人 日本薬学会

雑誌

Chemical and Pharmaceutical Bulletin (ISSN:00092363)

巻号頁・発行日

vol.62, no.6, pp.586-590, 2014

被引用文献数

6

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Salusin-β has been detected in numerous mammalian tissues and has been shown to have various effects on the cardiovascular system. In this study, we showed that salusin-β exhibited potent antibacterial activity against Gram-positive microorganisms such as Bacillus subtilis NBRC 3513, Bacillus megaterium ATCC 19213, Staphylococcus aureus NBRC 12732, and Staphylococcus epidermidis NBRC 12933. A cytoplasmic membrane-depolarizing assay using the DiSC3(5) dye revealed that the addition of 4 nmol/mL of salusin-β caused the leakage of fluorescence dye from Staphylococcus aureus NBRC 12732. The antimicrobial potency and circular dichroism (CD) spectroscopy of five analogs related to salusin-β were examined to determine structure-function relationships in its N- and C-terminal regions. The results obtained suggest that the N-terminal sequences of the salusin-β molecule are important for the expression of the potent antimicrobial activity of this peptide. A profile corresponding to that of the α-helix conformation was observed in the salusin-β solution.

著者

大嶽 さと子 多川 則子 吉田 俊和 オオタケ サトコ タガワ ノリコ ヨシダ トシカズ Ohtake Satoko Tagawa Noriko Yoshida Toshikazu

出版者

大阪大学大学院人間科学研究科対人社会心理学研究室

雑誌

対人社会心理学研究 (ISSN:13462857)

巻号頁・発行日

vol.10, pp.179-185, 2010