著者

Ayako HASHIMOTO Taiho KAMBE

出版者

Brewing Society of Japan

雑誌

JOURNAL OF THE BREWING SOCIETY OF JAPAN (ISSN:09147314)

巻号頁・発行日

vol.106, no.12, pp.811-817, 2011 (Released:2017-03-28)

参考文献数

37

被引用文献数

4

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本解説では人体における亜鉛(Zn)の生理機能について概説し,Znの摂取の重要性及び最近発見されたZn輸送タンパク質(ZIP4)の機能についてわかりやすく解説していただいた。つづいて,著者らが実施した,各種の味噌を添加することによるZIP4発現促進に関する研究を中心にして,味噌成分がZIP4発現促進効果をもつ可能性,ならびに近年の都市型生活の中で懸念されるZn欠乏に対する発酵食品の有用性についての期待と展望を示していただいた。味噌の新しい機能性の概要と有用性についてわかりやすく解説していただいた。

著者

Yoshito Ikeda Mizuki Kawakami Yasuyuki Yamada Masayuki Munekane Kohei Sano Takahiro Mukai Taiho Kambe Nobukazu Shitan

出版者

The Pharmaceutical Society of Japan

雑誌

BPB Reports (ISSN:2434432X)

巻号頁・発行日

vol.6, no.3, pp.108-114, 2023 (Released:2023-06-14)

参考文献数

24

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Zinc is an essential nutrient with important biological functions, and its deficiency can lead to several diseases. The zinc transporter families, ZIP and ZNT, play essential roles in regulating zinc homeostasis and dynamics in the body and cells. Specifically, ZIP4 is the primary zinc transporter responsible for zinc absorption in the small intestine. Previous studies have shown that Panax ginseng (P. ginseng) extract can promote mouse Zip4 expression, and ginsenosides, including Rc and Re, enhance zinc uptake. However, the effects of other metabolites present in P. ginseng extract remain unclear. Therefore, we fractionated P. ginseng extract using chloroform, ethyl acetate, and n-butyl alcohol, and evaluated the effect of each fraction on zinc uptake using mouse Hepa and Hepa/MRE-Luc cells that stably expressed luciferase under the promoter of metal-responsive elements. Luciferase activity assays demonstrated that the chloroform (F1), ethyl acetate (F2), and n-butyl alcohol (F3) fractions increased cellular zinc uptake. In particular, F1 fraction was found to induce Zip4 mRNA and protein expressions, which significantly enhanced zinc uptake. Ginsenosides were mainly present in the F2 and F3 fractions, indicating that metabolites other than ginsenosides in the F1 fraction would enhance zinc uptake by inducing Zip4 mRNA and protein expressions. Our study offers novel insights into the molecular mechanisms underlying zinc uptake by P. ginseng.