著者

Nobuhiko Taguchi Minoru Yuriguchi Takuya Ando Ryosuke Kitai Hitomi Aoki Takahiro Kunisada

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.42, no.9, pp.1446-1449, 2019-09-01 (Released:2019-09-01)

参考文献数

16

被引用文献数

4 13

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During the process of skin regeneration following a skin injury, de novo hair follicle regeneration is initiated after wounding; however, these regenerated hairs are mostly unpigmented. The activation of epidermal melanocyte stem cells and their differentiation into regenerating hair follicles have been shown to be necessary for the pigmented hair regeneration after wounding. To determine the role of flavonoids in the regeneration of pigmented hairs, we applied the candidate flavonoids to the regenerating hair follicles after wounding and identified the flavonoid species that maximally induced pigmented hair regeneration. Flavonoids with two OH groups in the B-ring, such as sterubin, luteolin, and hydroxygenkwanin, showed promising effects in regenerating black pigmented hairs, while those with one OH group in the B-ring showed no significant change. Thus, flavonoids with two OH groups in their B-ring could be studied further as potential wound healing agents with the ability to regenerate pigmented hair.

著者

Takahiro KUNISADA Ken-ichi SHINODA

出版者

The Anthropological Society of Nippon

雑誌

Journal of the Anthropological Society of Nippon (ISSN:00035505)

巻号頁・発行日

vol.98, no.4, pp.471-482, 1990 (Released:2008-02-26)

参考文献数

17

被引用文献数

1 1

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縄文時代人骨3体から DNA を抽出し, PCR 法を用いてミトコンドリア DNAを増幅して解析を行い,その方法論的な問題点を考察した。今回用いた方法では,試料の保存状態•部位にかかわらず,ほぼ安定して DNA の分離と増幅が可能であり,その有効性が確かめられた。分離された DNA は,そのほとんどがヒト由来のものではなかったが, PCR 法によりヒトミトコンドリア由来の DNA の増幅を確認することができた。ミトコンドリア DNAのV 領域および D ループ領域に対する制限酵素を用いた解析では多型は検出できなかった。しかし増幅した V 領域の塩基配列を決定したところ,1個体では1箇所の変異が見出された。

著者

Nobuhiko Taguchi Takumi Homma Hitomi Aoki Takahiro Kunisada

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.43, no.10, pp.1451-1454, 2020-10-01 (Released:2020-10-01)

参考文献数

19

被引用文献数

2

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Hair follicular keratinocyte stem cells (HFKSC) which provide a functional niche for melanocyte stem cells (MSC) are the primary target of hair graying. However, little research has been done on anti-hair graying medicines targeting HFKSC. We focused on Eriodictyon angustifolium (Ea), which reduces human hair graying when applied topically. To investigate the protective effect of dietary Ea tea (EaT) on hair pigmentation, we used an acute mouse model of hair graying that mimics X-ray-induced DNA damage associated with age-related hair graying. Our results suggest that dietary EaT maintained the niche HFKSC function against X-ray-induced DNA damage and hair graying. These results indicate that dietary EaT may prevent age-related hair graying and serve as an anti-hair graying herbal medicine.