著者

Yoshikuni Kitamura Takafumi Watanabe Yukari Kato WeiWei Teoh Keiko Kataoka Yuki Yamaguchi Tomohiro Haruta Hideo Nishioka Kiyokazu Kametani

出版者

The Japanese Society for Horticultural Science

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.UTD-003, (Released:2018-06-19)

被引用文献数

1

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Information about mechanical reinforcement of decorative organs could help development of a novel technique that would give flowers with robust floral organs and broadly contribute to postharvest flower preservation. Hydrangeas (Hydrangea spp.) exhibit remarkable characteristics in terms of mechanical reinforcement of decorative sepals. Although decorative sepals at the flowering stage shrink when they are desiccated, decorative sepals after flowering maintain their shape even after desiccation. In this study, the lignifications of the vein cells in decorative sepals were analyzed using phloroglucinol/HCl-staining. The microstructure of the cell wall was analyzed using transmission electron microscopy (TEM). The three-dimensional structure of vein cells was analyzed using serial block-face scanning electron microscopy (SBF-SEM). Tubular- and spindle-shaped dead cells with a lignified pitted secondary cell wall were observed around the vessel elements in decorative sepals after flowering. These cells were observed as living cells without a secondary cell wall in the veins of decorative sepals at flowering and in fully expanded leaves. Further, 10 hydrangea cultivars were analyzed for development of mechanical reinforcement in vein cells, and some of them were compared by desiccation testing. Decorative sepals of a cultivar lacking those cells exhibited shrinkage after flowering when they were desiccated. In conclusion, dead cells with a lignified pitted secondary cell wall contribute to the reinforcement of veins in decorative sepals of hydrangeas and become sclerified parenchyma cells. Axial parenchyma sclerifying in veins after flowering is essential for robust hydrangea floral organs and represent a new type of mechanical reinforcement tissue in plant decorative floral organs.

著者

Yoshikuni Kitamura Sachiyo Ueno Hiroe Aizawa WeiWei Teoh

出版者

The Japanese Society for Horticultural Science

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.OKD-111, (Released:2017-12-20)

被引用文献数

5

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Two types of cut hydrangea flowers are marketed: Fresh-stage cut flowers (harvested just after the decorative sepals are completely colored before or during flowering) and antique-stage cut flowers (harvested when the decorative sepals become green and/or red after flowering). We investigated the differences in vase lives between fresh- and antique-stage cut flowers using 10 cultivars and 5 lines of hydrangea. The antique-stage cut flowers of ‘Endless Summer’, ‘Glowing Alps’, ‘Grünherz’, ‘Masja’, ‘Temari Ezo’, and line No. 2 exhibited significantly longer vase lives than their fresh-stage cut flowers. The difference in vase lives between the cut flowers of ‘Masja’ harvested at two different stages was approximately 60 days. We conducted further studies to elucidate the contribution of detailed differences in the harvest stages to differences in the vase lives using ‘Masja’ and ‘Xi’an’. Vase lives were compared among the cut flowers harvested at four different harvest stages that were divided based on flowering of florets and greening of the decorative sepals. The cut flowers of ‘Xi’an’ harvested at the decorative florets flowering- and decorative sepal greening-stages showed significantly longer vase lives than those harvested at the non-decorative floret flowering stage. The cut flowers of ‘Masja’ harvested at two harvest stages following the beginning of the decorative sepal greening stage exhibited significantly longer vase lives than those harvested at the other two harvest stages. A recutting treatment rescued the cut flowers harvested at the decorative sepal greening completed-stages that exhibited softening of the decorative sepals. This result suggested that the vase lives of cut hydrangea flowers were terminated by the disruption of water balance caused by vessel occlusion in the stem ends. In conclusion, a relatively longer vase life can be expected when cut hydrangea flowers are harvested after flowering of the decorative florets. However, we should note that cut flowers of some cultivars harvested at the decorative floret flowering stage may exhibit relatively short vase lives. Treatments that suppress vessel occlusion may be effective for lengthening the vase lives of cut hydrangea flowers.