- 著者
- Elnaz NAKHAEI Ko TAKEHARA Hikari SATO Khadijah ZAI Akihiro KISHIMURA Takeshi MORI Yoshiki KATAYAMA
- 出版者
- The Japan Society for Analytical Chemistry
- 雑誌
- Analytical Sciences (ISSN:09106340)
- 巻号頁・発行日
- vol.34, no.4, pp.501-504, 2018-04-10 (Released:2018-04-10)
- 参考文献数
- 23
- 被引用文献数
- 2 5
Therapeutic peptides and diagnostic agents with their molecular size below the renal clearance threshold suffer from short blood circulation time. Here, we report a novel design of peptide-based ligand with a strong binding affinity to human serum albumin (HSA), which can be used as a tag to extend the blood circulation of small-size molecules. We designed ligands with dual alkyl groups connected with a negatively charged spacer. The ligands showed both higher binding affinity to HSA and a higher retention in mice blood than that of a single alkylated peptide.
- 著者
- Haitao FENG Akihiro KISHIMURA Takeshi MORI Yoshiki KATAYAMA
- 出版者
- The Japan Society for Analytical Chemistry
- 雑誌
- Analytical Sciences (ISSN:09106340)
- 巻号頁・発行日
- vol.36, no.10, pp.1279-1283, 2020-10-10 (Released:2020-10-10)
- 参考文献数
- 28
- 被引用文献数
- 3
In the early stage of tumor development, tumor-associated macrophages (TAM) works to suppress tumor growth by secreting soluble factors including nitric oxide (NO). L-Arginine (Arg) is a substrate of nitric oxide synthase (NOS) expressed in TAM. Here we examined whether NO produced from Arg by macrophages works to enhance the effect of the anti-cancer drug, doxorubicin (Dox) by using a co-culture system of cancer cells with macrophages. By employing colorimetric analyses methods (Griess Reagent and Cell Counting kit-8), we found that NO produced from Arg by co-cultured macrophages could enhance the cytotoxic effect of Dox to cancer cells. Moreover, we found that augmentation is affected by the order of the addition of Arg and Dox. A prior addition of Arg to Dox and simultaneous addition showed the same enhancement effect, but a prior addition of Dox to Arg abolished the augmentation. This suggests that the co-administration of Arg with Dox would be an effective treatment to improve chemo-therapies.
- 著者
- Takuro Niidme Masanori Fujii Naotoshi Nakashima Yoshiki Katayama Yasuro Niidome
- 出版者
- (社)日本化学会
- 雑誌
- Chemistry Letters (ISSN:03667022)
- 巻号頁・発行日
- pp.150258, (Released:2015-04-17)
- 被引用文献数
- 11
Gold nanorods were intravenously injected into tumor-bearing mice. The distribution of gold in tumor tissue sections was mapped by imaging mass spectrometry (IMS) using a MALDI mass spectrometer. IMS images of tumor tissue sections indicated that the gold nanorods were not distributed throughout tumor tissues but were localized in vasculature-rich areas.