著者

Akira S. Hirao Atsushi Kumata Toshihito Takagi Yoshito Sasaki Takashi Shigihara Eiichi Kimura Shingo Kaneko

出版者

The Mycological Society of Japan

雑誌

Mycoscience (ISSN:13403540)

巻号頁・発行日

vol.63, no.3, pp.79-87, 2022-05-31 (Released:2022-05-31)

参考文献数

45

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Pholiota microspora (“nameko” in Japanese) is one of the most common edible mushrooms, especially in Japan, where sawdust-based cultivation is the most dominant method accounting for 99% of the production. The current strains for sawdust cultivation in Japan are considered to have been derived from a single wild strain collected from Fukushima, Japan, implying that commercial nameko mushrooms are derived from a severe genetic bottleneck. We tested this single founder hypothesis by developing 14 microsatellite markers for P. microspora to evaluate the genetic diversity of 50 cultivars and 73 wild strains isolated from across Japan. Microsatellite analysis demonstrated that sawdust-cultivated strains from Japan were significantly less genetically diverse than the wild strains, and the former displayed a significant bottleneck signature. Analyzing the genetic relationships among all genotypes also revealed that the sawdust-cultivated samples clustered into one monophyletic subgroup. Moreover, the sawdust-cultivated samples in Japan were more closely related than full-sibs. These results were consistent with the single founder hypothesis that suggests that all commercial nameko mushrooms produced in Japan are descendants of a single ancestor. Therefore, we conclude that cultivated P. microspora originated from a single domestication event that substantially reduced the diversity of commercial nameko mushrooms in Japan.

著者

Akira S. Hirao Atsushi Kumata Toshihito Takagi Yoshito Sasaki Takashi Shigihara Eiichi Kimura Shingo Kaneko

出版者

The Mycological Society of Japan

雑誌

Mycoscience (ISSN:13403540)

巻号頁・発行日

pp.MYC570, (Released:2022-05-20)

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Pholiota microspora (“nameko” in Japanese) is one of the most common edible mushrooms, especially in Japan, where sawdust-based cultivation is the most dominant method accounting for 99% of the production. The current strains for sawdust cultivation in Japan are considered to have been derived from a single wild strain collected from Fukushima, Japan, implying that commercial nameko mushrooms are derived from a severe genetic bottleneck. We tested this single founder hypothesis by developing 14 microsatellite markers for P. microspora to evaluate the genetic diversity of 50 cultivars and 73 wild strains isolated from across Japan. Microsatellite analysis demonstrated that sawdust-cultivated strains from Japan were significantly less genetically diverse than the wild strains, and the former displayed a significant bottleneck signature. Analyzing the genetic relationships among all genotypes also revealed that the sawdust-cultivated samples clustered into one monophyletic subgroup. Moreover, the sawdust-cultivated samples in Japan were more closely related than full-sibs. These results were consistent with the single founder hypothesis that suggests that all commercial nameko mushrooms produced in Japan are descendants of a single ancestor. Therefore, we conclude that cultivated P. microspora originated from a single domestication event that substantially reduced the diversity of commercial nameko mushrooms in Japan.

著者

佐々木 義登 ササキ ヨシト Yoshito SASAKI

雑誌

二松学舎大学人文論叢

巻号頁・発行日

vol.79, pp.126-143, 2007-10

著者

Yoshito Sasaki Yasuo Ishii Hironobu Abe Katsuaki Mitachi Takayoshi Watanabe Tadafumi Niizato

出版者

一般社団法人 園芸学会

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.MI-132, (Released:2016-08-11)

被引用文献数

3

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To examine the translocation of radiocesium scattered by the Fukushima Daiichi nuclear power plant accident that occurred in March 2011 to the Japanese chestnut, we investigated the autoradiography and radiocesium concentration in each part of Japanese chestnuts. The Japanese chestnut fruit has a thin skin between the kernel (cotyledons) and shell; the kernel of the fruit is edible. The 137Cs concentration in each part of the fruit was found to be almost the same at about 1.0 × 104 Bq·kg−1 DW, as well as leaves. The radiocesium concentration in chestnut weevil larvae found on the fruit was approximately one-seventh of that in the kernel.