著者
Tadasuke Ohnishi Mitsumasa Hida Yukio Nakamura Chikamune Wada
出版者
The Society of Physical Therapy Science
雑誌
Journal of Physical Therapy Science (ISSN:09155287)
巻号頁・発行日
vol.30, no.3, pp.474-478, 2018 (Released:2018-03-02)
参考文献数
23
被引用文献数
2

[Purpose] It is clinically important to accurately evaluate the alignment of hindfoot alignment from the body surface in weight-bearing position. In general, measurement is carried out using a goniometer, but the measurement with goniometer requires experience, and the reliability in this measurement is also in question. Therefore, it is necessary to establish a clinical measurement method to replace the goniometer. As a solution, we devised a hindfoot alignment measurement method with laser beam (HAML), which measures hindfoot alignment along the body surface using a laser beam. This study aimed to study the correlation between the measurement result along the body surface with laser or goniometer and that of radiography with regard to hindfoot alignment. [Subjects and Methods] Subjects included 23 individuals with knee osteoarthritis. Their hindfoot alignment using radiography and goniometry were measured. The correlation between the measurement result along the body surface with or without laser and radiography was investigated. [Results] The correlation between HAML and radiography was better than that between HAML and goniometry. [Conclusion] The correlation between radiography and HAML for measuring hindfoot alignment was good. This finding implies that HAML can be used as a screening test or epidemiological survey in regional medicine.
著者
Naoki HAYASHI Satoru MONZEN Koichi ITO Tsuyoshi FUJIOKA Yukio NAKAMURA Ikuo KASHIWAKURA
出版者
Journal of Radiation Research 編集委員会
雑誌
Journal of Radiation Research (ISSN:04493060)
巻号頁・発行日
vol.53, no.2, pp.195-201, 2012 (Released:2012-04-17)
参考文献数
28
被引用文献数
6

The present study aimed to estimate the clonogenic and differentiation potential of induced pluripotent stem (iPS) cells exposed to ionizing radiation. Compared with mouse hematopoietic stem/progenitor cells, iPS cells were less sensitive to radiation. To examine the effect of ionizing radiation on the early differentiation pathway of iPS cells, we assessed embryoid body (EB) formation. Although EB formation was observed at all radiation doses, EB diameter decreased in a radiation dose-dependent manner. At the same time, we analyzed the expression of genes specific to differentiation in the initial iPS cells and cells of EB. The expression of the endoderm marker Afp increased remarkably in cells of EB derived from non-irradiated iPS cells; however, in irradiated cells, this expression significantly decreased in a radiation dose-dependent manner. Further, the expressions of the pluripotent stem cell markers Nanog and Oct-4 and the early mesoderm marker Brachyury significantly decreased. The results of the present study suggest that radiosensitivity with regard to gene expression differs at various stages in the early differentiation pathways of iPS cells that lead to the formation of the 3 germ layers; the sensitivity is the highest in the genes expressed during the differentiation pathways of iPS cells, leading to the formation of the endoderm.