- 著者
- Sho Yamamoto Yutaka Yamagiwa Zentaro Inaba Takashi Handa
- 出版者
- The Japanese Society for Horticultural Science
- 雑誌
- The Horticulture Journal (ISSN:21890102)
- 巻号頁・発行日
- vol.87, no.1, pp.115-123, 2018 (Released:2018-02-06)
- 参考文献数
- 33
- 被引用文献数
- 2 2
Lilium japonicum Thunb. which has pink or white-colored funnel-like flowers, is distributed in Kyushu, Shikoku, and the western part of Honshu, the main island of Japan. L. auratum Lindl. which has large white flowers with yellow central stripes and red spots, is distributed in the eastern part of Honshu. Natural hybridization of these two species has only been found on the Izu Peninsula of Honshu. However, details of the variation and hybridity of the interspecific hybrid population of these species on this peninsula remain unknown. In the present study, we conducted a morphological examination using 43, 21, and 90 individuals of L. japonicum, L. auratum, and the putative hybrid, respectively, from six, four, and ten populations of the Izu Peninsula, respectively. In addition, we performed simple sequence repeat (SSR) analysis using 47, 41, and 106 individuals of L. japonicum, L. auratum, and the putative hybrid, respectively, from six, four, and ten populations, respectively. Putative hybrid populations that resembled L. japonicum in morphology and SSR profile were found in the southern to eastern part of the peninsula, whereas those that resembled L. auratum and those exhibiting an intermediate morphology and SSR profile were found in the southern part of the peninsula. Large morphological variations exist in putative hybrid in the southern population, and interspecific hybridization has occurred in the southern and eastern populations. These results suggest that the center of natural hybridization is located in the southern part of the Izu Peninsula.
- 著者
- Hiroyuki Katsuoka Naoya Hamabe Chiemi Kato Susumu Hisamatsu Fujio Baba Motohiro Taneishi Toshiyuki Sasaki Atsushi Ikegaya Zentaro Inaba
- 出版者
- Japanese Society for Plant Biotechnology
- 雑誌
- Plant Biotechnology (ISSN:13424580)
- 巻号頁・発行日
- vol.39, no.2, pp.93-100, 2022-06-25 (Released:2022-06-25)
- 参考文献数
- 41
- 被引用文献数
- 1
To introduce useful characteristics such as fragrance into Argyranthemum frutescens (L.) and to expand the variation, we conducted crosses using A. frutescens as the seed parent and Chamaemelum nobile (L.) All. as the pollen parent. All the tested cross combinations between the three strains of A. frutescens and one strain of C. nobile produced embryos, and healthy plants were obtained by ovule culture. The obtained plantlets had a white ray floret, and the leaf shape was intermediate to those of the parents. The individuals obtained from this cross were subjected to two methods to determine hybridity: flow cytometry analyses and cleaved amplified polymorphic sequence (CAPS) markers. For the CAPS marker, we selected the internal transcribed spacer (ITS) region, which is highly variable among the genera, as the region to be amplified. We selected restriction enzymes BmgT120 I and Afl II, which selectively cut common sequences in the genus Argyranthemum, based on the sequence analysis of one parent strain each of A. frutescens and C. nobile and alignment with known sequences of related species. Flow cytometry analyses and CAPS markers revealed that the individuals obtained from the cross between A. frutescens and C. nobile are intergeneric hybrids. In addition, these established methods were capable of quickly and reliably identifying hybrids between A. frutescens and C. nobile. This report shows for the first time that crossbreeding between A. frutescens (seed parent) and C. nobile (pollen parent) is possible, and further development of Argyranthemum breeding, such as the expansion of variation by intergeneric crosses, is expected.