著者

Wu Yuluo Hinenoya Atsushi Taguchi Takashi NAGITA Akira SHIMA Kensuke TSUKAMOTO Teizo SUGIMOTO Norihiko ASAKURA Masahiro YAMASAKI Shinji

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.72, no.5, pp.589-597, 2010

被引用文献数

27

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Shiga toxin-producing <i>Escherichia coli</i> (STEC) isolated from Japan were investigated for the distribution of virulence genes. A total of 232 STEC strains including 171 from cattle and 61 from human were examined for the occurrence of genes responsible for bacterial adhesions to intestine, e.g., <i>eae</i> (intimin, <i>E. coli </i>attaching and effacing), <i>saa</i> (STEC autoagglutinating adhesin), <i>iha</i> (<i>irgA</i> homologue adhesin), <i>efa1</i> (<i>E. coli</i> factor for adherence), <i>lpfA</i><i>_O113</i> (long polar fimbriae), and <i>ehaA</i> (EHEC autotransporter) by colony hybridization assay. Similarly, the presence of toxigenic <i>cdt</i> (cytolethal distending toxin), and <i>subAB</i> (subtilase cytotoxin) genes were also checked. Among cattle isolates, 170, 163, 161, 155, 112 and 84 were positive for <i>lpfA</i><i>_O113</i> (99%), <i>ehaA</i> (95%), <i>iha</i> (94%), <i>saa</i> (91%), <i>subAB</i> (65%), and <i>cdt-V</i> (49%), respectively, while 2 were positive for <i>eae</i> (1.2%) and <i>efa1</i> (1.2%) each. In case of human isolates, 60, 59, 58 and 58 were positive for <i>ehaA</i> (98%), <i>iha</i> (97%), <i>efa1</i> (95%), and <i>eae</i> (95%), respectively, while 11, 2, 2, and 1 were positive for <i>lpfA</i><i>_O113</i> (18%), <i>saa</i> (3.3%), <i>cdt-V</i> (3.3%), and <i>subAB</i> (1.6%), respectively. Therefore, in human STEC isolates <i>efa1</i> and <i>eae</i> whereas in cattle isolates <i>saa</i>, <i>lpfA</i><i>_O113</i>, <i>cdt-V</i> and <i>subAB</i> were prevalent. These data indicate differential occurrence of some pathogenic genes in human and cattle originated STEC strains in Japan.<br>

著者

Hidenori KABEYA Kazuhiko OHASHI Misao ONUMA

出版者

JAPANESE SOCIETY OF VETERINARY SCIENCE

雑誌

Journal of Veterinary Medical Science (ISSN:09167250)

巻号頁・発行日

vol.63, no.7, pp.703-708, 2001 (Released:2001-08-07)

参考文献数

69

被引用文献数

40 68

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Bovine leukemia virus (BLV) is a type C retrovirus infecting bovine B cells and causing enzootic bovine leukosis. Since it takes long periods to develop the disease, it is believed that BLV and host immune responses are closely related. In this review, the accumulated data showing close relationship between BLV and host immune responses are summarized in 4 sections. First, we discuss the role of cell-mediated immunity in protecting hosts from BLV infection. Second, several reports showing the relationship between the disease progression and the change of cytokine profiles are summarized. In the third section, we have focused on tumor necrosis factor α (TNFα) and its two types of receptors, and the possible involvement of TNF α in the BLV-induced leukemogenesis is discussed. The expression of TNF α has been shown to be regulated by major histocompatibility complex (MHC) haplotype. The resistance to BLV infection is supposed to be established by some innate factors, which are closely related to MHC haplotype. Finally, we propose that a breeding strategy based on the MHC haplotype could be a good approach to control BLV infection. This review includes some recent data from us and other groups.