著者

中根 崇智

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.64, no.2, pp.125-131, 2022-05-31 (Released:2022-06-01)

参考文献数

16

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The past decade has seen revolutionary progress in single particle analysis with cryo-electron microscopes. Steady improvement in resolution has culminated in the reports of atomic resolution(1.2 Å)structures of apoferritin in 2020. This review, as a follow-up to my previous review in 2018, summarizes hardware and software improvements that enabled this milestone achievement. A quantitative analysis of the relationship between the number of particles and the resolution is presented and its practical implication in data collection strategies is discussed.