著者
Yu Nakajima Takashi Tsukamoto Yohei Kumagai Yoshitoshi Ogura Tetsuya Hayashi Jaeho Song Takashi Kikukawa Makoto Demura Kazuhiro Kogure Yuki Sudo Susumu Yoshizawa
出版者
Japanese Society of Microbial Ecology · The Japanese Society of Soil Microbiology
雑誌
Microbes and Environments (ISSN:13426311)
巻号頁・発行日
vol.33, no.1, pp.89-97, 2018 (Released:2018-03-29)
参考文献数
43
被引用文献数
17

Light-driven ion-pumping rhodopsins are widely distributed among bacteria, archaea, and eukaryotes in the euphotic zone of the aquatic environment. H+-pumping rhodopsin (proteorhodopsin: PR), Na+-pumping rhodopsin (NaR), and Cl−-pumping rhodopsin (ClR) have been found in marine bacteria, which suggests that these genes evolved independently in the ocean. Putative microbial rhodopsin genes were identified in the genome sequences of marine Cytophagia. In the present study, one of these genes was heterologously expressed in Escherichia coli cells and the rhodopsin protein named Rubricoccus marinus halorhodopsin (RmHR) was identified as a light-driven inward Cl− pump. Spectroscopic assays showed that the estimated dissociation constant (Kd,int.) of this rhodopsin was similar to that of haloarchaeal halorhodopsin (HR), while the Cl−-transporting photoreaction mechanism of this rhodopsin was similar to that of HR, but different to that of the already-known marine bacterial ClR. This amino acid sequence similarity also suggested that this rhodopsin is similar to haloarchaeal HR and cyanobacterial HRs (e.g., SyHR and MrHR). Additionally, a phylogenetic analysis revealed that retinal biosynthesis pathway genes (blh and crtY) belong to a phylogenetic lineage of haloarchaea, indicating that these marine Cytophagia acquired rhodopsin-related genes from haloarchaea by lateral gene transfer. Based on these results, we concluded that inward Cl−-pumping rhodopsin is present in genera of the class Cytophagia and may have the same evolutionary origins as haloarchaeal HR.

言及状況

外部データベース (DOI)

Twitter (18 users, 30 posts, 65 favorites)

吉澤さんにご紹介いただいた、好塩菌っぽい性質を持つ、海洋細菌のロドプシンは https://t.co/rMCfdA8hMY に概要を載せています。 元論文は、 https://t.co/2MvlLjoUBo https://t.co/WkzkeHhRv6
ま、Chitinophagaは、自分の論文の系統樹の中に入れてるんですけどねw https://t.co/2MvlLjFXDo
既に見解は示されているものの、当論文におけるFig.4やTable1、Fig6のRubricoccus, Ruvrivirgaの活性測定、分光学的解析はNakajima et al. 2018 https://t.co/jnmKoIuJeF で示しているので、color turningの話やとしても、引用されいいのではと読んでいて思いました。 非常に残念です。 https://t.co/iBUiuobx2W
シアノロドプシンの隣、シアノバクテリアルハロロドプシンは、論文自体で触れられているけど、実は、Nakajima et al. 2018で報告した海洋細菌の配列も含まれているクラスターなんで、みんな!忘れないでね!RmHR https://t.co/2MvlLjFXDo https://t.co/gjVVyXWwEP
このグループがどんなロドプシンを持ってるかというのと16S rRNA系統樹については、 https://t.co/jnmKoIuJeF こういうのがありまして。 (系統樹とかロドプシンの情報、もう少し更新したデータはNCBI内なども含めアップデートはされているけど)
Our paper is here. https://t.co/uMCwXDFLVu
Presence of a Haloarchaeal Halorhodopsin-Like Cl− Pump in Marine Bacteria https://t.co/hguBIrtcgc https://t.co/ZaMzcZ3iAj

収集済み URL リスト