著者

秋山 遼太 中安 大 梅基 直行 村中 俊哉 水谷 正治

出版者

一般社団法人植物化学調節学会

雑誌

植物の生長調節 (ISSN:13465406)

巻号頁・発行日

vol.52, no.2, pp.92-98, 2017

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<p>Steroidal glycoalkaloids (SGAs) are toxic specialized metabolites that are found in Solanaceae. Potato (<i>Solanum tuberosum</i>) contains the SGAs α-solanine and α-chaconine, which are biosynthesized from cholesterol. Several biosynthetic genes including <i>SSR2</i> and two cytochrome P450 genes (<i>CYP72A188</i> and <i>CYP72A208</i>) have been identified, and the transgenic potato plants silencing these biosynthetic genes showed SGA-reduced phenotypes. Here we summarize our recent results and strategy towards metabolic engineering of potato accumulating pharmaceutically useful compounds by genome editing. <i>CYP88B1</i>, which is involved in a later step of the SGA biosynthetic pathway with unknown catalytic function, is co-ordinately expressed with the SGA biosynthetic genes. We applied CRISPR/Cas9 system to knockout potato <i>CYP88B1</i>. The <i>CYP88B1</i>-knockout potatoes showed no accumulation of SGAs, and furthermore the corresponding amounts of steroidal saponins were accumulated in the knockout potatoes.</p>