著者

秋山 遼太 中安 大 水谷 正治

出版者

公益社団法人 日本薬学会

雑誌

ファルマシア (ISSN:00148601)

巻号頁・発行日

vol.57, no.8, pp.726-730, 2021 (Released:2021-08-01)

参考文献数

18

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ジャガイモはステロイドグリコアルカロイド(SGA)と呼ばれる有毒なα-ソラニンを含有している。SGAは少量であれば不快なえぐみを示す程度だが,多量に摂取した場合は食中毒を引き起こす。トマトにも有毒なSGAであるα-トマチン が葉や花、未熟果実に蓄積しているが、赤く熟した果実には有毒SGAは蓄積しておらず、食中毒を引き起こすことはない。本稿では、SGA生合成遺伝子同定に関する最近の研究の進展を解説するとともに,それらの知見を生かした有毒SGAを生産しないジャガイモの作出について紹介する.

著者

秋山 遼太 中安 大 梅基 直行 村中 俊哉 水谷 正治

出版者

一般社団法人植物化学調節学会

雑誌

植物の生長調節 (ISSN:13465406)

巻号頁・発行日

vol.52, no.2, pp.92-98, 2017

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<p>Steroidal glycoalkaloids (SGAs) are toxic specialized metabolites that are found in Solanaceae. Potato (<i>Solanum tuberosum</i>) contains the SGAs α-solanine and α-chaconine, which are biosynthesized from cholesterol. Several biosynthetic genes including <i>SSR2</i> and two cytochrome P450 genes (<i>CYP72A188</i> and <i>CYP72A208</i>) have been identified, and the transgenic potato plants silencing these biosynthetic genes showed SGA-reduced phenotypes. Here we summarize our recent results and strategy towards metabolic engineering of potato accumulating pharmaceutically useful compounds by genome editing. <i>CYP88B1</i>, which is involved in a later step of the SGA biosynthetic pathway with unknown catalytic function, is co-ordinately expressed with the SGA biosynthetic genes. We applied CRISPR/Cas9 system to knockout potato <i>CYP88B1</i>. The <i>CYP88B1</i>-knockout potatoes showed no accumulation of SGAs, and furthermore the corresponding amounts of steroidal saponins were accumulated in the knockout potatoes.</p>

著者

秋山 遼太 中安 大 梅基 直行 村中 俊哉 水谷 正治

出版者

一般社団法人植物化学調節学会

雑誌

植物の生長調節 (ISSN:13465406)

巻号頁・発行日

vol.52, no.2, pp.92-98, 2017 (Released:2018-01-12)

参考文献数

33

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Steroidal glycoalkaloids (SGAs) are toxic specialized metabolites that are found in Solanaceae. Potato (Solanum tuberosum) contains the SGAs α-solanine and α-chaconine, which are biosynthesized from cholesterol. Several biosynthetic genes including SSR2 and two cytochrome P450 genes (CYP72A188 and CYP72A208) have been identified, and the transgenic potato plants silencing these biosynthetic genes showed SGA-reduced phenotypes. Here we summarize our recent results and strategy towards metabolic engineering of potato accumulating pharmaceutically useful compounds by genome editing. CYP88B1, which is involved in a later step of the SGA biosynthetic pathway with unknown catalytic function, is co-ordinately expressed with the SGA biosynthetic genes. We applied CRISPR/Cas9 system to knockout potato CYP88B1. The CYP88B1-knockout potatoes showed no accumulation of SGAs, and furthermore the corresponding amounts of steroidal saponins were accumulated in the knockout potatoes.