著者

梅基 直行

出版者

公益社団法人 日本農芸化学会

雑誌

化学と生物 (ISSN:0453073X)

巻号頁・発行日

vol.53, no.12, pp.843-849, 2015-11-20 (Released:2016-11-20)

参考文献数

41

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グリコアルカロイドは,管理を誤ることでジャガイモに増加・蓄積し,ヒトや家畜に中毒を起こす潜在的な危険物質である.従来の育種ではグリコアルカロイドをなくすことができないとされてきた.近年,この生合成にかかわる遺伝子が同定されつつある.われわれと競合グループの成果,今後の見通しについて解説する.

著者

秋山 遼太 中安 大 梅基 直行 村中 俊哉 水谷 正治

出版者

一般社団法人植物化学調節学会

雑誌

植物の生長調節 (ISSN:13465406)

巻号頁・発行日

vol.52, no.2, pp.92-98, 2017

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<p>Steroidal glycoalkaloids (SGAs) are toxic specialized metabolites that are found in Solanaceae. Potato (<i>Solanum tuberosum</i>) contains the SGAs α-solanine and α-chaconine, which are biosynthesized from cholesterol. Several biosynthetic genes including <i>SSR2</i> and two cytochrome P450 genes (<i>CYP72A188</i> and <i>CYP72A208</i>) have been identified, and the transgenic potato plants silencing these biosynthetic genes showed SGA-reduced phenotypes. Here we summarize our recent results and strategy towards metabolic engineering of potato accumulating pharmaceutically useful compounds by genome editing. <i>CYP88B1</i>, which is involved in a later step of the SGA biosynthetic pathway with unknown catalytic function, is co-ordinately expressed with the SGA biosynthetic genes. We applied CRISPR/Cas9 system to knockout potato <i>CYP88B1</i>. The <i>CYP88B1</i>-knockout potatoes showed no accumulation of SGAs, and furthermore the corresponding amounts of steroidal saponins were accumulated in the knockout potatoes.</p>

著者

梅基 直行 水谷 正治 村中 俊哉

出版者

公益社団法人 日本農芸化学会

雑誌

化学と生物 (ISSN:0453073X)

巻号頁・発行日

vol.56, no.8, pp.566-572, 2018-07-20 (Released:2019-07-20)

参考文献数

32

著者

秋山 遼太 中安 大 梅基 直行 村中 俊哉 水谷 正治

出版者

一般社団法人植物化学調節学会

雑誌

植物の生長調節 (ISSN:13465406)

巻号頁・発行日

vol.52, no.2, pp.92-98, 2017 (Released:2018-01-12)

参考文献数

33

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Steroidal glycoalkaloids (SGAs) are toxic specialized metabolites that are found in Solanaceae. Potato (Solanum tuberosum) contains the SGAs α-solanine and α-chaconine, which are biosynthesized from cholesterol. Several biosynthetic genes including SSR2 and two cytochrome P450 genes (CYP72A188 and CYP72A208) have been identified, and the transgenic potato plants silencing these biosynthetic genes showed SGA-reduced phenotypes. Here we summarize our recent results and strategy towards metabolic engineering of potato accumulating pharmaceutically useful compounds by genome editing. CYP88B1, which is involved in a later step of the SGA biosynthetic pathway with unknown catalytic function, is co-ordinately expressed with the SGA biosynthetic genes. We applied CRISPR/Cas9 system to knockout potato CYP88B1. The CYP88B1-knockout potatoes showed no accumulation of SGAs, and furthermore the corresponding amounts of steroidal saponins were accumulated in the knockout potatoes.