著者

小川 哲弘 赤地 拓澄 増田 貴久子 山口 宏二 矢澤 一良 高橋 守 河岸 洋和

出版者

日本菌学会

雑誌

日本菌学会大会講演要旨集

巻号頁・発行日

vol.50, pp.106, 2006

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In recent years, the number of osteoporosis patients is increasing. Various foods were screened for inhibition activity of osteoclast formation and we found that <I>Agrocybe chaxingu</I> showed potent inhibition activity. In this research, we tried to purify the active principle(s) from <I>Agrocybe chaxingu</I>.Powder of dried fruiting bodies of the mushroom was extract with CH<SUB>2</SUB>Cl<SUB>2</SUB>, followed by EtOAc and EtOH. After removing each solvent, each fraction was tested for the inhibition activity. Scince the CH<SUB>2</SUB>Cl<SUB>2</SUB> soluble part showed the strongest inhibition activity, the fraction was fractionated by flash column chromatography, preparative TLC, and HPLC with a C30 column. As a result, we obtained an active compound (8.1 mg) and determined its structure. This compound strongly inhibited osteoclast formation without no cytotoxicity.