著者

鈴木 智大 小川 哲弘 阿部 暢男 赤地 拓澄 増田 貴久子 小山 智之 矢澤 一良 河岸 洋和

出版者

天然有機化合物討論会実行委員会

雑誌

天然有機化合物討論会講演要旨集

巻号頁・発行日

vol.49, pp.383-388, 2007

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Osteoporosis is caused by an imbalance between bone resorption and bone formation, which results in bone loss and fractures after mineral flux. Osteoclast-like multinucleated cells can be differentiated in vitro from co-cultures of mouse bone marrow cells and osteoblastic cells by treatment with osteotropic factors, 1α,25-dihydroxyvitamin D3 (1α,25(OH)_2D_3) and prostaglandin E2 (PGE2). During screening for osteoclast-formation suppressing effects of the extracts of various mushrooms by using the assay, we found very strong activity in the extract of the mushroom Agrocybe chaxingu. Therefore, an attempt was made to isolate the active principles from mushroom and to determine their structures. Powder of the dried fruiting bodies of Agrocybe chaxingu was extracted with CH_2Cl_2, EtOAc and then EtOH. The CH_2Cl_2-soluble fraction only showed the suppressing activity. After repeated chromatography of the fraction, compounds 1 and 2 were purified as the active principles. Osteoclast differentiation was estimated by TRAP-(+) multinucleated cell formation. The addition of compound 1and 2 (3.1μg/ml, 6.8mM) reduced the number of TRAP-(+) multinucleated cells to 66% and 0%, respectively.

著者

小川 哲弘 赤地 拓澄 増田 貴久子 山口 宏二 矢澤 一良 高橋 守 河岸 洋和

出版者

日本菌学会

雑誌

日本菌学会大会講演要旨集

巻号頁・発行日

vol.50, pp.106, 2006

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In recent years, the number of osteoporosis patients is increasing. Various foods were screened for inhibition activity of osteoclast formation and we found that <I>Agrocybe chaxingu</I> showed potent inhibition activity. In this research, we tried to purify the active principle(s) from <I>Agrocybe chaxingu</I>.Powder of dried fruiting bodies of the mushroom was extract with CH<SUB>2</SUB>Cl<SUB>2</SUB>, followed by EtOAc and EtOH. After removing each solvent, each fraction was tested for the inhibition activity. Scince the CH<SUB>2</SUB>Cl<SUB>2</SUB> soluble part showed the strongest inhibition activity, the fraction was fractionated by flash column chromatography, preparative TLC, and HPLC with a C30 column. As a result, we obtained an active compound (8.1 mg) and determined its structure. This compound strongly inhibited osteoclast formation without no cytotoxicity.