著者
濱舘 直史 瀬戸 加代子 矢澤 一良
出版者
日本補完代替医療学会
雑誌
日本補完代替医療学会誌 (ISSN:13487922)
巻号頁・発行日
vol.11, no.1, pp.67-74, 2014 (Released:2014-05-01)
参考文献数
12
被引用文献数
2

黒酢は,日本をはじめとする東アジアを中心に伝統のある発酵食品であり,様々な健康機能を持っていることが報告されている.本試験では,軽度肥満成人に黒酢含有食品を 12 週間摂取させるとともに毎日 10 分間程度の運動を併用させることで,体脂肪の減少効果およびエネルギー代謝への効果について,プラセボ食品を対照としたダブルブラインド法にて検討した.その結果,黒酢含有食品摂取群では,ヒップ周囲長の減少傾向,消費エネルギーが脂肪メインになるとともに運動時のエネルギー消費量が大きくなる傾向,脂肪量が多いほど脂肪量を減少させる効果が大きいことが示された.また,12 週間の黒酢含有食品の安全性について問題は見られず,安全に摂取できる食品であることが確認された.以上のことから,適切な運動と黒酢含有食品の摂取は,メタボリックシンドロームの改善に寄与することが示唆された.
著者
入来 常徳 渡辺 和朗 矢澤 一良
出版者
家畜栄養生理研究会
巻号頁・発行日
vol.40, no.2, pp.111-128, 1996 (Released:2011-03-05)
著者
矢澤 一良
出版者
缶詰技術研究会
雑誌
食品と容器 (ISSN:09112278)
巻号頁・発行日
vol.44, no.10, pp.552-558, 2003
被引用文献数
1
著者
鈴木 智大 小川 哲弘 阿部 暢男 赤地 拓澄 増田 貴久子 小山 智之 矢澤 一良 河岸 洋和
出版者
天然有機化合物討論会実行委員会
雑誌
天然有機化合物討論会講演要旨集
巻号頁・発行日
vol.49, pp.383-388, 2007

Osteoporosis is caused by an imbalance between bone resorption and bone formation, which results in bone loss and fractures after mineral flux. Osteoclast-like multinucleated cells can be differentiated in vitro from co-cultures of mouse bone marrow cells and osteoblastic cells by treatment with osteotropic factors, 1α,25-dihydroxyvitamin D3 (1α,25(OH)_2D_3) and prostaglandin E2 (PGE2). During screening for osteoclast-formation suppressing effects of the extracts of various mushrooms by using the assay, we found very strong activity in the extract of the mushroom Agrocybe chaxingu. Therefore, an attempt was made to isolate the active principles from mushroom and to determine their structures. Powder of the dried fruiting bodies of Agrocybe chaxingu was extracted with CH_2Cl_2, EtOAc and then EtOH. The CH_2Cl_2-soluble fraction only showed the suppressing activity. After repeated chromatography of the fraction, compounds 1 and 2 were purified as the active principles. Osteoclast differentiation was estimated by TRAP-(+) multinucleated cell formation. The addition of compound 1and 2 (3.1μg/ml, 6.8mM) reduced the number of TRAP-(+) multinucleated cells to 66% and 0%, respectively.
著者
小川 哲弘 赤地 拓澄 増田 貴久子 山口 宏二 矢澤 一良 高橋 守 河岸 洋和
出版者
日本菌学会
雑誌
日本菌学会大会講演要旨集
巻号頁・発行日
vol.50, pp.106, 2006

In recent years, the number of osteoporosis patients is increasing. Various foods were screened for inhibition activity of osteoclast formation and we found that <I>Agrocybe chaxingu</I> showed potent inhibition activity. In this research, we tried to purify the active principle(s) from <I>Agrocybe chaxingu</I>.Powder of dried fruiting bodies of the mushroom was extract with CH<SUB>2</SUB>Cl<SUB>2</SUB>, followed by EtOAc and EtOH. After removing each solvent, each fraction was tested for the inhibition activity. Scince the CH<SUB>2</SUB>Cl<SUB>2</SUB> soluble part showed the strongest inhibition activity, the fraction was fractionated by flash column chromatography, preparative TLC, and HPLC with a C30 column. As a result, we obtained an active compound (8.1 mg) and determined its structure. This compound strongly inhibited osteoclast formation without no cytotoxicity.
著者
矢澤 一良 影山 治夫
出版者
Japan Oil Chemists' Society
雑誌
油化学 (ISSN:18842003)
巻号頁・発行日
vol.40, no.10, pp.974-978, 1991-10-20 (Released:2009-10-16)
参考文献数
27
被引用文献数
5 7

Docosahexaenoic Acid (DHA) has important physiological activities, such as reduction of the plasma cholesterol and triglyceride, lowering effect of systolic blood pressure, anti-inflamatory and anti-tumor activities, etc. Recent studies also strongly suggest that DHA plays a key role in the brain and retina. DHA commonly exists in fish oil obtained from sardines, mackerels, menhadens, etc. Usually, however, it contains DHA at a relatively low level (5-10%) and many other polyunsaturated fatty acids, and thus isolation of pure DHA from fish oil is quite difficult.Recently, we found that (1) orbital fat of a bonito (Katsuwonus) and a tuna (Thunnus) fish was an excellent source of DHA, (2) DHA content in the total fatty acids of the orbital fat isolated from a bonito and a tuna fish was 42% and 30%, respectively, and (3) DHA existed as triglyceride in both cases.Purification of DHA was examined after ethanolysis of orbital fat oil obtained from a tuna fish, and resulted in 95%-99% purity of DHA-ethyl ester by a new method using Ag ion. Thus, finding of the excellent source and the purification procedure will greatly accelerate the research on DHA.