- 著者
-
小川 哲弘
赤地 拓澄
増田 貴久子
山口 宏二
矢澤 一良
高橋 守
河岸 洋和
- 出版者
- 日本菌学会
- 雑誌
- 日本菌学会大会講演要旨集
- 巻号頁・発行日
- vol.50, pp.106, 2006
In recent years, the number of osteoporosis patients is increasing. Various foods were screened for inhibition activity of osteoclast formation and we found that <I>Agrocybe chaxingu</I> showed potent inhibition activity. In this research, we tried to purify the active principle(s) from <I>Agrocybe chaxingu</I>.Powder of dried fruiting bodies of the mushroom was extract with CH<SUB>2</SUB>Cl<SUB>2</SUB>, followed by EtOAc and EtOH. After removing each solvent, each fraction was tested for the inhibition activity. Scince the CH<SUB>2</SUB>Cl<SUB>2</SUB> soluble part showed the strongest inhibition activity, the fraction was fractionated by flash column chromatography, preparative TLC, and HPLC with a C30 column. As a result, we obtained an active compound (8.1 mg) and determined its structure. This compound strongly inhibited osteoclast formation without no cytotoxicity.