著者

平川 周作 中島 淳 松木 昌也 古賀 敬興 秦 弘一郎 柏原 学 古閑 豊和 石間 妙子 宮脇 崇 金子 洋平 志水 信弘 松本 源生 石橋 融子

出版者

一般社団法人 日本環境化学会

雑誌

環境化学 (ISSN:09172408)

巻号頁・発行日

vol.30, pp.125-132, 2020 (Released:2020-09-18)

参考文献数

28

被引用文献数

1

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In this study, we examined a fish survey method using environmental DNA (eDNA) metabarcoding in a river, and the relationship between the fish survey results and water quality detected in eDNA was analyzed. eDNA surveys and fishing surveys were conducted in summer and winter, targeting three sites on three rivers in Fukuoka prefecture. Our comparison of riffles and pools and our examination of the appropriate places to collect eDNA samples revealed that the detected fish species were not completely consistent between the two kinds of surveys and there was no ecological features common to detected fish species. Therefore, sampling at multiple locations is expected to reduce detectable fish dropouts. More fish species were detected in the eDNA survey than in the collection survey at all times. However, the results also suggested that the DNA of fish species living upstream may remain at sampling site, and benthic fish species tend to be difficult to detect by eDNA surveys. In addition, the influence of water quality on the characteristics of the survey site were analyzed, since marine species were detected in some river eDNA surveys. The results suggested that domestic drainage is likely to affect the characteristics in winter, when the amount of water is small, and the change in concentration of linear alkylbenzene sulfonic acid and its salts and the change of the ratio of chloride ions may be used as an index of domestic drainage.

著者

古閑 豊和 高橋 浩司

出版者

一般社団法人 日本環境化学会

雑誌

環境化学 (ISSN:09172408)

巻号頁・発行日

vol.33, pp.74-82, 2023 (Released:2023-08-30)

参考文献数

23

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We developed a novel mass spectrometric method using atmospheric pressure gas chromatography-tandem mass spectrometry (APGC/MS/MS) with nitrogen as the carrier gas to quantify simazine and thiobencarb under the regulatory environmental quality standards for water pollution. We modified the previous official method and optimized the sample preparation procedure using solid-phase extraction (SPE) cartridges. Our study demonstrated good recoveries for simazine and thiobencarb in all SPE cartridges, with the percentage recoveries ranging from 70% to 120%. Of the examined SPE cartridge, a marked measurement precision with the least variation was achieved on Smart-SPE PLS3-20 (PLS3) cartridge, which is packed with nitrogen-containing divinylbenzene methacrylate. Consequently, we selected the PLS3 SPE cartridge for the sample preparation in this method. Moreover, The method's detection and quantification limit values for simazine and thiobencarb in river and seawater samples were one order of magnitude lower than those of the environmental quality standard values. Furthermore, simazine and thiobencarb were identified and quantified with excellent separation, minimizing potential interferences.

著者

古閑 豊和

出版者

日本環境毒性学会

雑誌

環境毒性学会誌 (ISSN:13440667)

巻号頁・発行日

vol.27, no.S1, pp.S93-S104, 2023-11-10 (Released:2023-11-10)

参考文献数

27

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A method for evaluating the combined effects of chemicals is the whole-mixture approach, which evaluates a water sample containing a mixture of several chemicals. In this approach, chemical analysis is important to identify the cause of toxicity. However, among the numerous chemicals present in wastewater and river water, it is difficult to identify the toxic chemicals using conventional chemical analysis methods. Therefore, a comprehensive analytical method capable of detecting many chemicals is required. This study focused on target screening methods for organic contaminants, especially in chemical analysis; introduced a gas chromatograph-mass spectrometer (GC-MS) database that could identify nearly 1000 organic compounds; and developed a rapid screening method. Furthermore, the combined effects on water samples using the target screening methods and biological response tests were evaluated.