著者

平川 周作 中島 淳 松木 昌也 古賀 敬興 秦 弘一郎 柏原 学 古閑 豊和 石間 妙子 宮脇 崇 金子 洋平 志水 信弘 松本 源生 石橋 融子

出版者

一般社団法人 日本環境化学会

雑誌

環境化学 (ISSN:09172408)

巻号頁・発行日

vol.30, pp.125-132, 2020 (Released:2020-09-18)

参考文献数

28

被引用文献数

1

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In this study, we examined a fish survey method using environmental DNA (eDNA) metabarcoding in a river, and the relationship between the fish survey results and water quality detected in eDNA was analyzed. eDNA surveys and fishing surveys were conducted in summer and winter, targeting three sites on three rivers in Fukuoka prefecture. Our comparison of riffles and pools and our examination of the appropriate places to collect eDNA samples revealed that the detected fish species were not completely consistent between the two kinds of surveys and there was no ecological features common to detected fish species. Therefore, sampling at multiple locations is expected to reduce detectable fish dropouts. More fish species were detected in the eDNA survey than in the collection survey at all times. However, the results also suggested that the DNA of fish species living upstream may remain at sampling site, and benthic fish species tend to be difficult to detect by eDNA surveys. In addition, the influence of water quality on the characteristics of the survey site were analyzed, since marine species were detected in some river eDNA surveys. The results suggested that domestic drainage is likely to affect the characteristics in winter, when the amount of water is small, and the change in concentration of linear alkylbenzene sulfonic acid and its salts and the change of the ratio of chloride ions may be used as an index of domestic drainage.

著者

古賀 敬興 平川 周作 石橋 融子 堀 就英

出版者

公益社団法人 日本食品衛生学会

雑誌

食品衛生学雑誌 (ISSN:00156426)

巻号頁・発行日

vol.62, no.3, pp.100-104, 2021-06-25 (Released:2021-07-02)

参考文献数

8

被引用文献数

1

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マイクロ波分解装置を用いたミネラルウォーター類以外の清涼飲料水のスズ,ヒ素,鉛分析法の検討と性能評価を実施した.3元素を通して,真度93~100%,併行精度0.7~6.1 RSD%,室内精度0.9~8.6 RSD%であった.スズの試料調製においては,マイクロ波分解処理後に硫酸を添加する操作を追加することによって添加回収率および再現性の向上が確認された.また,本手法の適用性を検証するため,性状の異なる5種類の清涼飲料水について添加回収試験を実施した結果,回収率は92~102%であった.本手法によって幅広いミネラルウォーター類以外の清涼飲料水の分解処理およびスズ,ヒ素,鉛の定量分析への適用が期待でき,さらに既開発法と比較して作業時間の短縮および作業の簡便化が実現できた.