著者

向井 友花 及川 勉

出版者

公益社団法人 日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

vol.136, no.10, pp.1335-1343, 2016 (Released:2016-10-01)

参考文献数

15

被引用文献数

1

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Tests on physiological functions of umami have been actively conducted and a need recognized for a high-performance quantification device that is simple and cost-effective, and whose use is not limited to a particular location or user. To address this need, Ajinomoto Co. and Tanita Corp. have jointly been researching and developing a simple device for glutamate measurement. The device uses L-glutamate oxidase immobilized on a hydrogen peroxide electrode. L-glutamate in the sample is converted to α-ketoglutaric acid, which produces hydrogen peroxide. Subsequently, the electrical current from the electrochemical reaction of hydrogen peroxide is measured to determine the L-glutamate concentration. In order to evaluate its basic performance, we used this device to measure the concentration of L-glutamate standard solutions. In a concentration range of 0-1.0%, the difference from the theoretical value was minimal. The coefficient of variation (CV) value of 3 measurements was 4% or less. This shows that the device has a reasonable level of precision and accuracy. The device was also used in trial measurements of L-glutamate concentrations in food. There was a good correlation between the results obtained using the developed device and those obtained with an amino acid analyzer; the correlation coefficient was R=0.997 (n=24). In this review, we demonstrate the use of our device to measure the glutamate concentration in miso soup served daily at a home for elderly people, and other foods and ingredients.

著者

佐藤 伸 向井 友花 嵯峨井 勝

雑誌

日本衞生學雜誌 (ISSN:00215082)

巻号頁・発行日

vol.63, no.2, 2008-03-01

著者

向井 友花

出版者

青森県立保健大学

雑誌

若手研究(B)

巻号頁・発行日

2009

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妊娠高血圧症候群の病態の軽減に対する植物性ポリフェノールの有効性を見いだすため、妊娠ラットに一酸化窒素(NO)合成酵素阻害剤を投与あるいはフルクトース(果糖)を過剰摂取させ、アズキポリフェノール摂取が血圧、酸化ストレス、糖・脂質代謝に及ぼす影響を検討した。その結果、アズキポリフェノール摂取はNO欠乏妊娠ラットに顕著な血圧上昇抑制効果は示さなかったが、腎臓のMn-SOD発現を増加させた。またフルクトース摂取妊娠ラットに認められた肝臓の脂質合成転写因子の発現上昇を一部抑制した。