著者

豊田 淑江 森田 育男 室田 誠逸

出版者

公益社団法人 日本薬理学会

雑誌

日本薬理学雑誌 (ISSN:00155691)

巻号頁・発行日

vol.107, no.3, pp.99-107, 1996 (Released:2007-02-06)

参考文献数

54

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Angiogenesis, a process of new blood vessel formation, is an integral part of development, wound repair and tumor growth. The formation of capillary networks requires a complex series of cellular events, in which endothelial cells locally degrade their basement membrane, migrate into the connective tissue stroma, proliferate at the migrating tip, elongate and organize into capillary loops. In response to angiogenic stimuli, endothelial cells in culture develop networks of capillarylike tubes. In this paper, we showed the relationship between angiogenesis and diseases, the assay systems of angiogenesis and the reports of angiogenesis published recently.

著者

森田 育男 室田 誠逸

出版者

公益社団法人 日本農芸化学会

雑誌

化学と生物 (ISSN:0453073X)

巻号頁・発行日

vol.21, no.3, pp.168-173, 1983-03-25 (Released:2009-05-25)

参考文献数

10

著者

室田 誠逸

出版者

日本炎症・再生医学会

雑誌

炎症 (ISSN:03894290)

巻号頁・発行日

vol.20, no.2, pp.103-105, 2000-03-29 (Released:2010-04-12)

著者

室田 誠逸 森田 育男

出版者

一般社団法人 日本動脈硬化学会

雑誌

動脈硬化 (ISSN:03862682)

巻号頁・発行日

vol.16, no.7, pp.925-928, 1988-12-01 (Released:2011-09-21)

参考文献数

2

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For the simple examination of the change in vascular permeability response of endothelial cells to various stimuli, we attempted to establish an in vitro experimental model using Boyden's chamber. This chamber has both upper and lower compartments with a partition filter between them. We succeeded in obtaining a monolayer of vascular endothelial cells on the surface of the filter by using a gelatin coated nucleopore filter. Both upper and lower compartments were filled with culture medium. When bovine serum albumin was added only to the upper compartment, the albumin was time-dependently leaked from the upper compartment to the lower compartment though the pores of the filter. The leakage was inhibited greatly when the surface of the filter was covered with entothelial cells.By monitoring the amount of albumin leaked into the lower compartment after the addition of various stimuli, such as histamine, serotonin, bradykinin, PAF (Platelet activating factor) etc., to the upper compartment, we could judge which stimulus could better influence vascular permeability. PAF was found to be the most active stimulus in increasing vascular permeability.During the course of the investigation, we found that such substances as having cytotoxic activity, i.e., lipid peroxides, antiserum in the presence of the complement etc., could also increase vascular permeability. Therefore, by measuring cell viability at the same time, this model can be used as an in vitro assay system for measuring vascular endothelial cell injury. This model may also be a useful in vitro assay system for the evaluation of cytoprotective drugs. By using this system we found that 15-HPETE (15-hydroxyeicosatetraenoic acid), one of the lipoxygenase metabolites of arachidonic acid, has a strong cytolitic activity on vascular endothelial cells and that MCI-186 (3-methyl-1-phenyl-2-pyrazolin-5-one) could prevent the cells from such injury.

著者

室田 誠逸

出版者

日本脂質栄養学会

雑誌

脂質栄養学 (ISSN:13434594)

巻号頁・発行日

vol.4, no.2, pp.135-135, 1995-08-11 (Released:2009-04-10)