著者

清河 信敬 恩田 恵子 高野 邦彦 藤本 純一郎 真部 淳 康 勝好 小原 明 林 泰秀 花田 良二 土田 昌宏

出版者

日本サイトメトリー学会

雑誌

Cytometry research : 日本サイトメトリー学会機関誌 (ISSN:09166920)

巻号頁・発行日

vol.20, no.2, pp.27-33, 2010-09-25

参考文献数

6

被引用文献数

1

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<p>Aim. We are in charge of the central diagnosis and cell preservation as a part of childhood acute lymphoblastic leukemia treatment study in Tokyo children's cancer study group. It is necessary to diagnose with a minimal quantity of specimen, to preserve leukemic cells effectively as possible. On the other hand, recent progress in multi-color flow cytometry enable to analyze cell marker of leukemia in more detail. We therefore we intended to perform a immuno-phenotypic diagnosis of childhood acute lymphoblastic leukemia by nine-color analysis with digital flow cytometer.</p><p>Methods. We examined cell markers of childhood acute lymphoblastic leukemia cells by nine-color analysis using digital flow cytometers. We decided the combination of the monoclonal antibodies for nine color analysis based on the recommendation of Japan Pediatric Leukemia/Lymphoma Study Group using commercially available fluorescence-laveled antibodies.</p><p>Results. Nine colors that we used in this study were fluorescein isothyocyanate, phycoerythrin (PE), phycoerythrin-Texas Red, Peridinin Chlorophyll Protein - cyanin (Cy) 5.5, PE-Cy7, allophycocyanin (APC), APC-Cy7, Pacific Blue, and Cascade Yellow. Using list mode compensation, nine color marker analysis of childhood leukemia was easy to perform.</p><p>Discussion. Although several problems need to be solved are present, nine-color analysis using digital flow cytometer is useful to obtain precise information of antigen expression as well as save precious specimen of childhood acute lymphoblastic leukemia.</p>