- 著者
-
清河 信敬
恩田 恵子
高野 邦彦
藤本 純一郎
真部 淳
康 勝好
小原 明
林 泰秀
花田 良二
土田 昌宏
- 出版者
- 日本サイトメトリー学会
- 雑誌
- Cytometry research : 日本サイトメトリー学会機関誌 (ISSN:09166920)
- 巻号頁・発行日
- vol.20, no.2, pp.27-33, 2010-09-25
- 参考文献数
- 6
- 被引用文献数
-
1
<p>Aim. We are in charge of the central diagnosis and cell preservation as a part of childhood acute lymphoblastic leukemia treatment study in Tokyo children's cancer study group. It is necessary to diagnose with a minimal quantity of specimen, to preserve leukemic cells effectively as possible. On the other hand, recent progress in multi-color flow cytometry enable to analyze cell marker of leukemia in more detail. We therefore we intended to perform a immuno-phenotypic diagnosis of childhood acute lymphoblastic leukemia by nine-color analysis with digital flow cytometer.</p><p>Methods. We examined cell markers of childhood acute lymphoblastic leukemia cells by nine-color analysis using digital flow cytometers. We decided the combination of the monoclonal antibodies for nine color analysis based on the recommendation of Japan Pediatric Leukemia/Lymphoma Study Group using commercially available fluorescence-laveled antibodies.</p><p>Results. Nine colors that we used in this study were fluorescein isothyocyanate, phycoerythrin (PE), phycoerythrin-Texas Red, Peridinin Chlorophyll Protein - cyanin (Cy) 5.5, PE-Cy7, allophycocyanin (APC), APC-Cy7, Pacific Blue, and Cascade Yellow. Using list mode compensation, nine color marker analysis of childhood leukemia was easy to perform.</p><p>Discussion. Although several problems need to be solved are present, nine-color analysis using digital flow cytometer is useful to obtain precise information of antigen expression as well as save precious specimen of childhood acute lymphoblastic leukemia.</p>