- 著者
-
服部 考成
福士 惠一
早川 真
湊 太郎
- 出版者
- 公益社団法人 日本分析化学会
- 雑誌
- 分析化学 (ISSN:05251931)
- 巻号頁・発行日
- vol.62, no.8, pp.737-741, 2013-08-05 (Released:2013-09-04)
- 参考文献数
- 12
We developed a capillary zone electrophoresis method with direct UV detection for the determination of inorganic anions (Cl−, NO2−, and NO3−), organic acids (oxalic acid, citric acid, and malic acid), and amino acids (aspartic acid and glutamic acid) in the common ice plant (Mesembryanthemum crystallinum L.). As the background electrolyte, a 20 mmol L−1 disodium hydrogenphosphate dodecahydrate solution adjusted to pH 10.6 was used with the addition of 0.001% (w/v) hexadimethrine bromide to reverse electroosmotic flow. The detection responses for the above analytes were improved by 3.5 – 580 fold in terms of the limits of detection (LODs, S/N = 3) in comparison with conventional indirect UV detection. The LODs for the analytes were 0.040 – 2.9 mg L−1. The values of the relative standard deviation (RSD, n = 4, intra-day) of the migration time and the peak area were, respectively, 0.081 – 0.43% and 0.28 – 9.3% when extract from the common ice plant was analyzed. Using the proposed procedure, Cl−, NO2−, NO3−, and oxalic acid in the common ice plant were detected within 9 min; citric acid, malic acid, aspartic acid, and glutamic acid were detected within 12 min.