著者
榊 利之 安田 佳織 西川 美宇 生城 真一
出版者
公益社団法人 日本薬学会
雑誌
YAKUGAKU ZASSHI (ISSN:00316903)
巻号頁・発行日
vol.138, no.3, pp.357-363, 2018-03-01 (Released:2018-03-01)
参考文献数
22
被引用文献数
2 9

Sesamin, derived from sesame seeds, is known to have various biological effects. Since some of these effects appear to be derived from its metabolites, the elucidation of sesamin metabolism is essential to understanding the molecular mechanism of its effects. In addition, it is important to clarify drug-sesamin interactions in order to address safety concerns, as some food factors are known to affect drug metabolism. Our previous studies revealed that sesamin was sequentially metabolized by cytochrome P450 (CYP) and UDP-glucuronosyltransferase or sulfotransferase. Whereas sesamin metabolism is mainly mediated by CYP2C9 in human liver, sesamin causes a mechanism-based inhibition (MBI) of CYP2C9. However, we found that the metabolite-intermediate complex between CYP2C9 and sesamin was unstable, and the effects of sesamin appeared to be minimal. To confirm this assumption, in vivo studies using rats were conducted. After the administration of sesamin to rats for 3 d, diclofenac (an NSAID) was administered to measure the time course of plasma concentration of diclofenac. No significant differences were observed in the diclofenac Cmax, Tmax, and AUC0-24 h between the group that was administered sesamin and the group that was not. Based on these results, it could be concluded that no significant interaction occurs in people who take sesamin supplements at a standard dose.
著者
木村 成伸 五十嵐 淑郎 生城 真一 千田 俊哉
出版者
茨城大学
雑誌
基盤研究(A)
巻号頁・発行日
2009

シアノバクテリアSynechocystis sp. PCC6803にAcidovorax sp. KKS102株ビフェニル分解酵素系遺伝子やラット肝臓シトクロムP4501A1遺伝子を導入することにより,ビフェニルやダイオキシンを貧栄養環境下で水酸化できる新規シアノバクテリア株を作製した。芳香族環境汚染物質のバイオレメディエーションにおけるシアノバクテリアの有用性が確認できた。