- 著者
- 藤原 博 石田 説而 島崎 義男 内藤 誠之郎 土谷 正和 松浦 脩治
- 出版者
- 公益社団法人 日本薬学会
- 雑誌
- YAKUGAKU ZASSHI (ISSN:00316903)
- 巻号頁・発行日
- vol.110, no.5, pp.332-340, 1990-05-25 (Released:2008-05-30)
- 参考文献数
- 17
- 被引用文献数
- 1 3
The amounts of endotoxin in commercial blood products were measured by the turbidimetric kinetic Limulus test with an ordinary reagent (LAL-HS) and a new endotoxinspecific reagent (LAL-ES). LAL-ES contains a sufficient amount of a water-soluble (1→3)-β-D-glucan derivative as a blocker of the (1→3)-β-D-glucan-mediated coagulation pathway in the reaction of the Limulus amebocyte lysate. The amounts of endotoxin in alubumin and globulin products measured with LAL-ES agreed with pyrogenic activities in rabbits, but those measured with LAL-HS did not. Added endotoxin in the blood products was well recovered with LAL-ES, but that in some products was excessively recovered with LAL-HS. The amounts of endotoxin in diphtheria-pertussis-tetanus combined vaccines measured with LAL-HS and LAL-ES agreed with the pyrogenic activities in rabbits. The results suggested the existence of a false-positive substance like β-glucan in the blood products but not in the vaccine. LAL-ES is more suitable for the detection of endotoxin in blood products than LAL-HS.
- 著者
- 岩佐 三郎 石田 説而 赤真 清人
- 出版者
- National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
- 雑誌
- Japanese Journal of Medical Science and Biology (ISSN:00215112)
- 巻号頁・発行日
- vol.38, no.2, pp.53-65, 1985 (Released:2010-03-19)
- 参考文献数
- 35
- 被引用文献数
- 3 3
Intracerebral injection of vaccine into the mouse induced swelling of the brain. The swelling reached the maximum in the intensity by day 1 and persisted for several days. A method for quantitative determination of the brain-swelling activity of the vaccine was developed. A positive regression coefficient was found only between the brain-swelling and the lymphocytosis-promoting activities. Such activity was no longer shown with the vaccine heat-treated for 30 min at 80 C, but it was restored upon addition of the lymphocytosis-promoting factor (LPF) that caused no brain swelling by itself. The activity, therefore, was ascribed to cooperation of LPF and a certain heat-stable component other than endotoxin contained by pertussis vaccine.