著者

木根 啓太 砂川 直輝 石田 卓也 五十嵐 圭日子 鮫島 正浩 金子 哲

出版者

一般社団法人 日本エネルギー学会

雑誌

バイオマス科学会議発表論文集 (ISSN:24238333)

巻号頁・発行日

vol.12, pp.157-158, 2017

---

<p>Two α-L-arabinofuranosidase genes classified in Glycoside Hydrolase (GH) family 51 from the basidiomycetes Phanerochaete chrysosporium and Flammulina velutipes were cloned, and the recombinant enzymes expressed in the yeast Pichia pastoris were characterized. When p-nitrophenyl-α-L-arabinofuranoside was used as a substrate, two enzymes showed acidic pH optima at pH 3. The two enzymes released L-arabinose from rye arabinoxylan, wheat arabinoxylan, sugar beet arabinan and debranched arabinan, but not from arabinogalactan.</p>