著者
木根 啓太 砂川 直輝 石田 卓也 五十嵐 圭日子 鮫島 正浩 金子 哲
出版者
一般社団法人 日本エネルギー学会
雑誌
バイオマス科学会議発表論文集 (ISSN:24238333)
巻号頁・発行日
vol.12, pp.157-158, 2017

<p>Two α-L-arabinofuranosidase genes classified in Glycoside Hydrolase (GH) family 51 from the basidiomycetes Phanerochaete chrysosporium and Flammulina velutipes were cloned, and the recombinant enzymes expressed in the yeast Pichia pastoris were characterized. When p-nitrophenyl-α-L-arabinofuranoside was used as a substrate, two enzymes showed acidic pH optima at pH 3. The two enzymes released L-arabinose from rye arabinoxylan, wheat arabinoxylan, sugar beet arabinan and debranched arabinan, but not from arabinogalactan.</p>
著者
中村 彰彦 石田 卓也 鮫島 正浩 五十嵐 圭日子
出版者
日本結晶学会
雑誌
日本結晶学会誌 (ISSN:03694585)
巻号頁・発行日
vol.57, no.1, pp.59-65, 2015-02-28 (Released:2015-03-05)
参考文献数
7

Structural analysis including hydrogen position is important for estimating the reaction mechanism of hydrolases. We applied neutron and X-ray structural analysis to a mysterious β-1,4 glucanase from a fungi Phanerochaete chrysosporium (PcCel45A). The joint analysis of neutron and X-ray structures showed that PcCel45A utilizing an imidic acid form of asparagine residue as a general base instead of typical acidic residues.