著者

藤橋 雅宏

出版者

日本結晶学会

雑誌

日本結晶学会誌 (ISSN:03694585)

巻号頁・発行日

vol.63, no.3, pp.222-223, 2021-08-31 (Released:2021-09-02)

参考文献数

3

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The recent developments of automated data collection and remote access technologies at synchrotron facility are highly remarkable. Here I describe our recent attempt to identify phosphates using their anomalous signals without visiting synchrotron facility. Crystals were screened using automated data collection with the X-ray at the wavelength of 1.0 Å. Then the solvent around the qualified crystals was removed by deep-UV laser ablation. The diffraction datasets were subsequently collected from the solvent-removed crystals using the X-ray at the wavelength of 2.7 Å. Anomalous Fourier map from the dataset collected with 2.7 Å X-ray was superposed on the electron density map calculated from the dataset collected with 1.0 Å X-ray. The two maps clearly indicated the positions of non-Carbon/Nitrogen/Oxygen atoms in the crystal. Our trial proved that considerable parts of the experiments in the synchrotron facility could be performed from the remote site.

著者

佐藤 努 藤橋 雅宏 品田 哲郎

出版者

新潟大学

雑誌

基盤研究(C)

巻号頁・発行日

2013-04-01

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ゲノムマイニングおよび基質許容性解析・多機能性解析から新規テルペンの発見や非天然型テルペンの創出を達成することができた。特に、オノセロイド(スクアレンの両末端環化によって生合成されるトリテルペン)合成酵素を初めて見いだすことができた。加えて、香料や媚薬として利用される龍涎香の主成分アンブレインの酵素合成に成功した。マイコバクテリア由来の新型酵素の発掘や新規酵素の触媒機構解析も順調に進んだ。