著者

Chinachoti Noppawan 遠藤 直之 園元 謙二 石崎 文彬

出版者

九州大学

雑誌

九州大学農学部紀要 (ISSN:00236152)

巻号頁・発行日

vol.42, no.3, pp.421-436, 1998-03

被引用文献数

2

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Continuous fermentation was introduced to improve the nisin Z productivity of Lactococcus lactis IO-1. Free cells showed a good productivity at the dilution rate of 0.1 h^<-1>. However, nisin Z production was affected by cell wash-out at the dilution rate of 0.2h^<-1>. Continuous fermentation with the cells adsorbed on ENTG-3800 gel beads displayed an improvement in productivity at higher dilution rates. No enhancement of nisin Z production was observed during continuous fermentation at a high cell density employing a hydrophobic hollow fiber membrane. The polyolefm membrane adsorbed the produced nisin Z too much. Continuous fermentation at a high cell density employing a ceramic membrane displayed a good nisin Z productivity at high dilution rates. Nisin Z productivity could be increased if a ceramic membrane with an adequate and effective filtration area is employed. Nisin Z was separated from the fermentation broth using various kinds of adsorbents including Amberlite IR-120B, CM Sephadex C-25, Celite, and Sep-Pak cartridges. The Sep-Pak (tC_18, C_18, C_8, and tC_2) cartridges showed a substantial capacity for nisin Z adsorption. A moderate reversed-phase column, a Sep-Pak C, cartridge, was applied to integrate nisin Z fermentation with a ceramic membrane and product separation system. Nisin Z productivity was enhanced by the integration of the nisin Z adsorption cartridge. This result indicates the possibility of continuous fermentation with the integrated bioreactor system followed by high nisin Z productivity.