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著者
石崎 文彬
出版者
公益社団法人 日本農芸化学会
雑誌
化学と生物 (ISSN:0453073X)
巻号頁・発行日
vol.48, no.7, pp.503-505, 2010-07-01 (Released:2011-09-07)
参考文献数
1
著者
Etmy Herawati 石崎 文彬
出版者
九州大学
雑誌
九州大学農学部紀要 (ISSN:00236152)
巻号頁・発行日
vol.42, no.3, pp.449-461, 1998-03
被引用文献数
1

Pediococcus sp. ISK-1, isolated in our laboratory, has been found to inhabit in well-aged Nukadoko which is believed to have lasted for 360 years. We compared the influence of ethanol between Lactobacillus homohiochii JCM 1199' and strain ISK-l and found that the growth of strain ISK-1 was seriously inhibited by ethanol. However, fermentation of ISK-1 was strongly stimulated by Yamahai-moto Sake and Sakekasu (Sake Filter Cake). Yamahai-moto Sake is obtained from traditional brewing, therefore, it was possible that Sakekasu would have been used for the first Nukadoko preparation at that time. Addition of 8.0% NaCI and 2.0% Sakekasu in MRS broth resulted in substantially improving the cell population and lactate formation. Based on those facts, it is presumed that the first Nukadoko may have been prepared by the supplementation of Sakekasu, Soy souce, and Salt with selective pressure resulting in enrichment of strain ISK-1 of the bacteriocin producer.
著者
吉村 研治 大森 庸子 吉松 隆夫 田中 賢二 石崎 文彬
出版者
日本水産學會
雑誌
日本水産学会誌 (ISSN:00215392)
巻号頁・発行日
vol.62, no.6, pp.897-903, 1996-11-15
参考文献数
6
被引用文献数
7 7

In a high density culture of rotifer (beyond the levels of 10<sup>3</sup>ind./m<i>l</i>), the amount of aeration needed to maintain an optimum dissolved oxygen (DO) level increases. In order to investigate the effect of aeration on the population growth of rotifer, a series of experiments were carried out. The S-type rotifer <i>Brachionus rotundiformis</i> was cultured at high density using 10<i>l</i> vessels under different aeration conditions. Air or oxygen gas was introduced into the culture media through two types of sparger which formed different sizes of bubbles, at aeration rates from 0.1 to 2.0vvm. The bubble-size from the two kinds of sparger were estimated from the value of <i>KLa</i> (volumetric oxygen transfer coefficient). When the aeration rate was high or the ceramic sparger that formed smaller bubbles was used, DO in the culture system remained at a high level. However, under these conditions, the growth of the rotifer population was seriously inhibited due to very high aeration rates and small bubbles, possibly as a result of foaming separation. When oxygen gas was introduced into the culture system, the density of rotifer was higher than that when air was introduced. Therefore, it is recommended to introduce high-purity oxygen gas for a high density culture to avoid any growth inhibition and to maintain optimum DO.
著者
Chinachoti Noppawan 遠藤 直之 園元 謙二 石崎 文彬
出版者
九州大学
雑誌
九州大学農学部紀要 (ISSN:00236152)
巻号頁・発行日
vol.42, no.3, pp.421-436, 1998-03
被引用文献数
2

Continuous fermentation was introduced to improve the nisin Z productivity of Lactococcus lactis IO-1. Free cells showed a good productivity at the dilution rate of 0.1 h^<-1>. However, nisin Z production was affected by cell wash-out at the dilution rate of 0.2h^<-1>. Continuous fermentation with the cells adsorbed on ENTG-3800 gel beads displayed an improvement in productivity at higher dilution rates. No enhancement of nisin Z production was observed during continuous fermentation at a high cell density employing a hydrophobic hollow fiber membrane. The polyolefm membrane adsorbed the produced nisin Z too much. Continuous fermentation at a high cell density employing a ceramic membrane displayed a good nisin Z productivity at high dilution rates. Nisin Z productivity could be increased if a ceramic membrane with an adequate and effective filtration area is employed. Nisin Z was separated from the fermentation broth using various kinds of adsorbents including Amberlite IR-120B, CM Sephadex C-25, Celite, and Sep-Pak cartridges. The Sep-Pak (tC_18, C_18, C_8, and tC_2) cartridges showed a substantial capacity for nisin Z adsorption. A moderate reversed-phase column, a Sep-Pak C, cartridge, was applied to integrate nisin Z fermentation with a ceramic membrane and product separation system. Nisin Z productivity was enhanced by the integration of the nisin Z adsorption cartridge. This result indicates the possibility of continuous fermentation with the integrated bioreactor system followed by high nisin Z productivity.