著者

黒岩 崇久

出版者

素粒子論グループ 素粒子論研究 編集部

雑誌

素粒子論研究 (ISSN:03711838)

巻号頁・発行日

vol.64, no.5, pp.171-228, 1982-02-20 (Released:2017-10-02)

著者

黒岩 崇久

出版者

素粒子論グループ 素粒子論研究 編集部

雑誌

素粒子論研究 (ISSN:03711838)

巻号頁・発行日

vol.74, no.5, pp.53-64, 1987-02-20 (Released:2017-10-02)

著者

黒岩 崇久

出版者

素粒子論グループ 素粒子論研究 編集部

雑誌

素粒子論研究 (ISSN:03711838)

巻号頁・発行日

vol.86, no.5, pp.49-79, 1993-02-20 (Released:2017-10-02)

著者

市川 創作 黒岩 崇

出版者

公益社団法人 日本薬学会

雑誌

YAKUGAKU ZASSHI (ISSN:00316903)

巻号頁・発行日

vol.128, no.5, pp.681-686, 2008 (Released:2008-05-01)

参考文献数

18

被引用文献数

2 3

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A vesicle is a compartment composed of lipid bilayer of amphiphilic molecules. The vesicle is applied to carriers of drugs, cosmetics and functional food ingredients in industries. Vesicles are also applied as a model for artificial cell membrane and expected as micro- and nano-reactors. They are generally prepared by the hydration of dry lipid film, but there is no method to prepare vesicles of a controlled size and high entrapment yield of hydrophilic materials inside them. In this article, a microchannel (MC) emulsification method was applied to prepare vesicles aimed at controlling the size and improving the entrapment yield. Firstly, monodisperse water-in-oil (W/O) emulsions were prepared by the MC emulsification method. In this process, hydrophilic materials to be entrapped were contained inside the water droplets of the emulsions. Keeping the water droplets frozen, the emulsifier was replaced by a bilayer-forming lipid mixture, and then the oil phase was evaporated. After hydration of lipid layers surrounding the water droplets, vesicles were formed. We call this preparation “lipid-coated ice droplet hydration method”. The final sizes of the prepared vesicles were comparable to the original emulsion droplet sizes. This means that the size of vesicles can be controlled by controlling the size of original water droplets of the W/O emulsions. Furthermore, calcein as a hydrophilic fluorescent marker and biopolymers, such as enzyme and polysaccharide, were entrapped into the internal water phases of vesicles. The method proposed in this study enables the formation of vesicles with a controlled size and high entrapment yields, potentially useful for expanding the application fields of vesicles as biocompatible carriers and micro- and nano-reactors for biochemical reactions.

著者

黒岩 崇 後藤 貴裕 河野 真也 横山 節雄 宮寺 庸造 中村 直人

出版者

一般社団法人電子情報通信学会

雑誌

電子情報通信学会技術研究報告. ET, 教育工学 (ISSN:09135685)

巻号頁・発行日

vol.97, no.464, pp.157-164, 1997-12-20

参考文献数

4

被引用文献数

8

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本学は, 付属学校のほとんどが遠隔にあるため, 大学と付属学校との連携した学生指導を行うことは難しい. そこで, ネットワークを利用し, 大学の教官が遠隔から研究授業を参観し, その後研究会に参加する形態を実施した. 本稿では, アンケートとインタビューから, 遠隔授業参観を行った際の有効性や課題について考察し, 遠隔参加型研究会のよりよい方向性を与える.