著者

Kenta Watanabe Chihiro Oda-Yamamizo Kimiyo Sage-Ono Akemi Ohmiya Michiyuki Ono

出版者

Japanese Society for Plant Biotechnology

雑誌

Plant Biotechnology (ISSN:13424580)

巻号頁・発行日

vol.34, no.4, pp.177-185, 2018-01-25 (Released:2018-01-27)

参考文献数

53

被引用文献数

8 7

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Japanese morning glory, Ipomoea nil, has several coloured flowers except yellow, because it can accumulate only trace amounts of carotenoids in the petal. To make the petal yellow with carotenoids, we introduced five carotenogenic genes (geranylgeranyl pyrophosphate synthase, phytoene synthase, lycopene β-cyclase and β-ring hydroxylase from Ipomoea obscura var. lutea and bacterial phytoene desaturase from Pantoea ananatis) to white-flowered I. nil cv. AK77 with a petal-specific promoter by Rhizobium (Agrobacterium)-mediated transformation method. We succeeded to produce transgenic plants overexpressing carotenogenic genes. In the petal of the transgenic plants, mRNA levels of the carotenogenic genes were 10 to 1,000 times higher than those of non-transgenic control. The petal colour did not change visually; however, carotenoid concentration in the petal was increased up to about ten-fold relative to non-transgenic control. Moreover, the components of carotenoids in the petal were diversified, in particular, several β-carotene derivatives, such as zeaxanthin and neoxanthin, were newly synthesized. This is the first report, to our knowledge, of changing the component and increasing the amount of carotenoid in petals that lack ability to biosynthesize carotenoids.

著者

Akemi Ohmiya Masaya Kato Takehiko Shimada Kenji Nashima Sanae Kishimoto Masayasu Nagata

出版者

The Japanese Society for Horticultural Science

雑誌

The Horticulture Journal (ISSN:21890102)

巻号頁・発行日

pp.UTD-R003, (Released:2019-03-12)

被引用文献数

26

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Carotenoids are isoprenoid pigments, which are widely distributed in nature. In fruits and flowers, carotenoids are responsible for bright yellow, orange, and red colors and provide a substrate to form flavor compounds, which attract pollinators and seed dispersers. In leaves, carotenoids play an essential role in photosynthesis. When carotenoids are ingested in the diet, they play a vital role in human nutrition and health as a precursor of vitamin A, antioxidants, and anti-inflammatory agents. It is therefore important to control carotenoid accumulation to improve the commercial value of horticultural crops. Carotenoid accumulation is regulated by flux through the carotenoid biosynthetic pathway, and also by degradation and sequestration into plastids, which function as sink organelles. These processes are mostly controlled at the transcriptional levels of relevant genes. In this review, we summarize recent advances in studies on the molecular mechanisms that regulate carotenoid accumulation in vegetables, fruits, and ornamental flowers.

著者

Akemi OHMIYA

出版者

Japan International Research Center for Agricultural Sciences

雑誌

Japan Agricultural Research Quarterly: JARQ (ISSN:00213551)

巻号頁・発行日

vol.45, no.2, pp.163-171, 2011 (Released:2012-01-19)

参考文献数

50

被引用文献数

19 62

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Carotenoids are yellow, orange, and red pigments that are widely distributed in nature. In plants, carotenoids play important roles in photosynthesis and furnishing flowers and fruits with distinct colors. While most plant leaves show similar carotenoid profiles, containing carotenoids essential for photosynthesis, the carotenoid composition of flower petals varies from species to species. In this review, I present a list of carotenoid composition in the flower petals of various plants and discuss the possible causes of qualitative diversity.