著者

Miya Urui Yasuyuki Yamada Akira Nakagawa Fumihiko Sato Hiromichi Minami Nobukazu Shitan

出版者

The Pharmaceutical Society of Japan

雑誌

Biological and Pharmaceutical Bulletin (ISSN:09186158)

巻号頁・発行日

vol.46, no.10, pp.1494-1497, 2023-10-01 (Released:2023-10-01)

参考文献数

15

---

Advancements in synthetic biology have facilitated the microbial production of valuable plant metabolites. However, constructing complete biosynthetic pathways within a single host organism remains challenging. To solve this problem, modular co-culture systems involving host organisms with partial pathways have been developed. We focused on Escherichia coli, a general host for metabolite production, and Pichia pastoris (Komagataella phaffii), a novel synthetic biology host due to its high expression of biosynthetic enzymes. Previously, we reported the co-culture of E. coli cells, which produce reticuline (an important intermediate for various alkaloids) from glycerol, with P. pastoris cells, which produce the valuable alkaloid stylopine from reticuline. However, Pichia cells inhibited E. coli growth and reticuline production. Therefore, we aimed to improve this co-culture system. We investigated the pre-culture time before co-culture to enhance E. coli growth and reticuline production. Additionally, we examined the optimal concentration of Pichia cells inoculated for co-culture and methanol addition during co-culture for the continuous expression of biosynthetic enzymes in Pichia cells. We successfully established an improved co-culture system that exhibited an 80-fold increase in productivity compared to previous methods. This enhanced system holds great potential for the rapid and large-scale production of various valuable plant metabolites.

著者

AKIRA NAKAGAWA HIROSHI TOMODA MAI VAN HAO KEIKO OKANO YUZURU IWAI SATOSHI OMURA

出版者

JAPAN ANTIBIOTICS RESEARCH ASSOCIATION

雑誌

The Journal of Antibiotics (ISSN:00218820)

巻号頁・発行日

vol.38, no.8, pp.1114-1115, 1985 (Released:2006-04-19)

参考文献数

6

被引用文献数

22 22

著者

Yoshio SUGIURA Akira NAKAGAWA Yuji MURAYAMA Ryuji FUNAYAMA Junko KUSAYAMA Toshiyuki GOSEKI Yoshihiro TAKIGUCHI Kanato SUZUKI

出版者

The Association of Japanese Geographers

雑誌

地理学評論 (ISSN:13479555)

巻号頁・発行日

vol.79, no.12, pp.743-746, 2006-10-01 (Released:2008-12-25)

著者

Yoshio SUGIURA Yuji MURAYAMA Akira NAKAGAWA Hitoshi HASEGAWA Yukihisa UCHIYAMA Toyohiko MIYAGI Hiroki YAMASHITA Masatomo UMITSU Hyoe TAMIYA

出版者

The Association of Japanese Geographers

雑誌

地理学評論 (ISSN:13479555)

巻号頁・発行日

vol.79, no.12, pp.751-754, 2006-10-01 (Released:2008-12-25)