著者

Chao ZHAO Kun ZHAO Xiaoyan LIU Yi-fan HUANG Bin LIU

出版者

Japanese Society for Food Science and Technology

雑誌

Food Science and Technology Research (ISSN:13446606)

巻号頁・発行日

vol.19, no.4, pp.661-667, 2013 (Released:2013-09-05)

参考文献数

35

被引用文献数

6 10

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Orthogonal experiment was used to optimize the extraction conditions of Flammulina velutipes mycelia polysaccharides (FvP). Four independent variables (ratio of water to raw material, initial pH value, extracting temperature, and extracting time) were taken into consideration. A yield of FvP of 2.19% was obtained under an optimized condition (ratio of water to material of 50:1, initial pH value of 6.0, extracting temperature of 85°C, and extracting time of 6 h). Subsequently, antioxidative properties of FvP-2 (crude polysaccharides) and FvP-3 (deproteinized polysaccharides) prepared from F. velutipes mycelia were evaluated by monitoring the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and hydroxyl radical scavenging activity, together with antitumor activity against the human hepatocellular carcinoma BEL-7402 cells. DPPH scavenging rate of 65.85% and hydroxyl radical scavenging rate of 71.24% were obtained at 200 μg/mL of FvP-3. The inhibition rate of BEL-7402 cells was up to 45% at 640 μg/mL of FvP-2. These results suggested that FvP possesses potent antioxidant and antitumor properties. The polysaccharide may be useful as a functional food additive and an antioxidant and antitumor agent.

著者

Bo Zhang Haidong Xu Juntao Wang Bin Liu Guodong Sun

出版者

バイオ&ソーシャル・サイエンス推進国際研究交流会

雑誌

BioScience Trends (ISSN:18817815)

巻号頁・発行日

vol.11, no.4, pp.406-417, 2017-08-31 (Released:2017-09-12)

参考文献数

80

被引用文献数

61

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Lumbar intervertebral disc herniation (LIDH), as the main contributor to low back pain and sciatica, imposes a heavy burden on both the individual and society. Non-operative treatment or conservative treatment has proven effective in alleviation of the symptoms of LIDH and are considered to be a first-line choice for most cases. Active lifestyle, physical therapy, complementary and alternative medicine therapy or Traditional Chinese medicine (TCM) therapy, and pharmacotherapy are routinely used as effective non-operative treatment for LIDH patients. However, how to choose one or several conservative treatments with higher efficacy, less side effects, minimal injury, and low cost is still a challenge for doctors and LIDH patients. Furthermore, there are some national characteristics for some conservative treatments in different countries, which bring difficulties for the widespread use of these methods. Here we initiated a search on the non-operative treatment especially TCM therapy for LIDH mainly using PubMed, Web of Science, China National Knowledge Internet (CNKI), and Chinese biomedicine database since the 1980s with no restriction of language. According to these related references, we gave a narrative review which emphasizes up-to-date knowledge regarding the effectiveness and safety of various conservative methods with special consideration for TCM therapy including acupuncture, autonomy, Chinese massage, and Chinese herbal medicines, for LIDH treatment. We hope this review will further contribute to an understanding of conservative treatment as an important choice for LIDH patients and provide useful information for the development of more effective conservative methods for LIDH treatment.

著者

Yi Li Zhengyi Zhang Xiangnan Zhou Rui Li Yan Cheng Bo Shang Yu Han Bin Liu Xiaodong Xie

出版者

日本循環器学会

雑誌

Circulation Journal (ISSN:13469843)

巻号頁・発行日

pp.CJ-17-0022, (Released:2017-07-26)

参考文献数

44

被引用文献数

7

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Background:The process of cardiomyocyte swelling involves changes of biomechanical properties and profiles of cellular genes. Although many genes have been proved to regulate cell edema of cardiomyocyte, the mechanisms involved in this event, as well as the biomechanical properties of swelling cell, remain unknown.Methods and Results:Whether histone deacetylase 1 (HDAC1) inhibition protects against hypoxia-induced H9c2 cardiomyocyte swelling is examined in this study. Hypoxia-induced changes in the biomechanical properties and cytoskeletal structure that are relevant to cell swelling were also determined. H9c2 cells were treated under a chemical hypoxia situation (cobalt chloride) with HDAC1 inhibition (chemical inhibitor or siRNA) for 5 h, followed by in vitro biological and mechanical characterization. The results showed that expression of HDAC1 instead of HDAC4 was upregulated by chemical hypoxia. HDAC1 inhibition protects H9c2 cells against chemical hypoxia-induced hypoxic injury and cell swelling. HDAC1 inhibition improved cell viability, decreased lactate dehydrogenase leakage, cell apoptosis, malondialdehyde concentration, cell volume, and particles on the cell surface, and increased superoxide dismutase activity. Moreover, chemical hypoxia induced a decrease of Young’s modulus, accompanied by alterations in the integrity of acetylated histone and organization of the cytoskeletal network. HDAC1 inhibition significantly reversed these processes.Conclusions:Based on the ideal physical model, HDAC1 inhibition protects against hypoxia-induced swelling in H9c2 cardiomyocytes through enhancing cell stiffness. Overall, HDAC1 is a potential therapeutic target for myocardial edema.

著者

Yi TANG Junchen JIANG Xiaofei WANG Chengchen HU Bin LIU Zhijia CHEN

出版者

The Institute of Electronics, Information and Communication Engineers

雑誌

IEICE TRANSACTIONS on Information and Systems (ISSN:09168532)

巻号頁・発行日

vol.E93-D, no.12, pp.3232-3242, 2010-12-01

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Multi-pattern matching is a key technique for implementing network security applications such as Network Intrusion Detection/Protection Systems (NIDS/NIPSes) where every packet is inspected against tens of thousands of predefined attack signatures written in regular expressions (regexes). To this end, Deterministic Finite Automaton (DFA) is widely used for multi-regex matching, but existing DFA-based researches have claimed high throughput at an expense of extremely high memory cost, so fail to be employed in devices such as high-speed routers and embedded systems where the available memory is quite limited. In this paper, we propose a parallel architecture of DFA called Parallel DFA (PDFA) taking advantage of the large amount of concurrent flows to increase the throughput with nearly no extra memory cost. The basic idea is to selectively store the underlying DFA in memory modules that can be accessed in parallel. To explore its potential parallelism we intensively study DFA-split schemes from both state and transition points in this paper. The performance of our approach in both the average cases and the worst cases is analyzed, optimized and evaluated by numerical results. The evaluation shows that we obtain an average speedup of 100 times compared with traditional DFA-based matching approach.