著者

Naofumi Yoshida Kengo Sasaki Daisuke Sasaki Tomoya Yamashita Hajime Fukuda Tomohiro Hayashi Tokiko Tabata Ro Osawa Ken-ichi Hirata Akihiko Kondo

出版者

Japan Atherosclerosis Society

雑誌

Journal of Atherosclerosis and Thrombosis (ISSN:13403478)

巻号頁・発行日

pp.47415, (Released:2018-12-27)

参考文献数

49

被引用文献数

24

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Aim: Bacteroides vulgatus and B. dorei have a protective effect against atherosclerosis, suggesting that expansion of these species in the gut microbiota could help patients with coronary artery disease (CAD). This study aimed to investigate the effect of resistant starch (RS) on the gut microbiota and its metabolites in fecal sample cultures from patients with CAD and individuals without CAD, using a single-batch fermentation system.Methods: Fecal samples from 11 patients with CAD and 10 individuals without CAD were fermented for 30 h with or without RS in the Kobe University Human Intestinal Microbiota Model (KUHIMM). Gut microbiota and the abundance of B. vulgatus and B. dorei were analyzed using 16S ribosomal ribonucleic acid (rRNA) gene sequencing and the quantitative polymerase chain reaction. Short-chain fatty acids were analyzed using high-performance liquid chromatography.Results: Gut microbial analysis showed significantly lower levels of B. vulgatus and B. dorei in the original fecal samples from patients with CAD, which was simulated after 30 h of fermentation in the KUHIMM. Although RS significantly increased the absolute numbers of B. vulgatus and B. dorei, and butyrate levels in CAD fecal sample cultures, the numbers varied among each patient.Conclusions: The effect of RS on gut microbiota and its metabolites in the KUHIMM varied between CAD and non-CAD fecal sample cultures. The KUHIMM may be useful for preclinical evaluations of the effects of RS on the gut microbiota and its metabolites.

著者

Kana Moriyama Daisuke Sasaki Yuichi Ono

出版者

Fuji Technology Press Ltd.

雑誌

Journal of Disaster Research (ISSN:18812473)

巻号頁・発行日

vol.13, no.6, pp.1007-1014, 2018-11-01 (Released:2018-11-01)

参考文献数

26

被引用文献数

22

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After the Sendai Framework for Disaster Risk Reduction is adopted, a global database as a tool to monitor disaster loss and damage databases is required. Several disaster loss and damage databases are in use globally. This paper aims to explore how the existing databases vary in three aspects of threshold, spatial resolution, and data quality control, as well as the limitations of the existing databases. We review previous studies comparing the existing global databases and extract the differences and limitations. The threshold of EM-DAT is clear, but its threshold results in ignoring small-scale disasters that DesInventar captures. The differences in disaster threshold create different pictures of disaster losses and/or risks. Regarding spatial resolution, only DesInventar provides disaster impact data at a municipal level, while others provide information at a country level. The limitations of the existing global database are categorized into four aspects, as follows: lack of disaggregated data, limited spatial coverage and resolution, insufficiency of completeness and reliability of data, and insufficient information on indirect loss. The implication from our findings is that, in order to complement the limitations of the existing disaster loss databases to use for decision making on disaster risk reduction, the following are required: cross-checking of data across different databases; complementary disaster loss data; and collection of an exhaustive and firsthand dataset with a transparent and internationally consistent methodology by policy makers.

著者

Mikio Ishiwatari Daniel P. Aldrich Daisuke Sasaki

出版者

Fuji Technology Press Ltd.

雑誌

Journal of Disaster Research (ISSN:18812473)

巻号頁・発行日

vol.18, no.5, pp.467-474, 2023-08-01 (Released:2023-08-01)

参考文献数

43

被引用文献数

1

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Whether national and local governments should assume larger financial responsibility for reducing disaster risk remains a controversial issue. Local governments lack resources to cover the full cost of managing mega-disasters and need assistance from higher level governments. However, national governments covering all costs may create moral hazards, discouraging local governments from investing in ex-ante measures. This study identifies national and local governments’ fiscal responsibility determinants for disaster management. Despite the differences between the federal system in the US and the centralized system in Japan, the two countries’ national governments share common practices. Both have continuously developed legislation to expand their financial responsibilities for relief and recovery efforts as disaster consequences have increased. We argue that despite major institutional differences in Japan’s unitary and the US federal government systems, both have expanded the areas covered by national assistance along with the amount over time. These findings bring with them recommendations for governments in an era of increasing extreme weather events due to climate change.

著者

Koichiro Miura Katsuhisa Matsuura Yu Yamasaki Itoyama Daisuke Sasaki Takuma Takada Yoshiyuki Furutani Emiko Hayama Masamichi Ito Seitaro Nomura Hiroyuki Morita Masashi Toyoda Akihiro Umezawa Kenji Onoue Yoshihiko Saito Hiroyuki Aburatani Toshio Nakanishi Nobuhisa Hagiwara Issei Komuro Tatsuya Shimizu

出版者

International Heart Journal Association

雑誌

International Heart Journal (ISSN:13492365)

巻号頁・発行日

vol.63, no.2, pp.338-346, 2022-03-30 (Released:2022-03-30)

参考文献数

35

被引用文献数

5

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Dilated cardiomyopathy (DCM) is caused by various gene variants and characterized by systolic dysfunction. Lamin variants have been reported to have a poor prognosis. Medical and device therapies are not sufficient to improve the prognosis of DCM with the lamin variants. Recently, induced pluripotent stem (iPS) cells have been used for research on genetic disorders. However, few studies have evaluated the contractile function of cardiac tissue with lamin variants. The aim of this study was to elucidate the function of cardiac cell sheet tissue derived from patients with lamin variant DCM. iPS cells were generated from a patient with lamin A/C (LMNA) -mutant DCM (LMNA p.R225X mutation). After cardiac differentiation and purification, cardiac cell sheets that were fabricated through cultivation on a temperature-responsive culture dish were transferred to the surface of the fibrin gel, and the contractile force was measured. The contractile force and maximum contraction velocity, but not the maximum relaxation velocity, were significantly decreased in cardiac cell sheet tissue with the lamin variant. A qRT-PCR analysis revealed that mRNA expression of some contractile proteins, cardiac transcription factors, Ca2+-handling genes, and ion channels were downregulated in cardiac tissue with the lamin variant.Human iPS-derived bioengineered cardiac tissue with the LMNA p.R225X mutation has the functional properties of systolic dysfunction and may be a promising tissue model for understanding the underlying mechanisms of DCM.

著者

Ryohei SHINOHARA Kengo SASAKI Jun INOUE Namiko HOSHI Itsuko FUKUDA Daisuke SASAKI Akihiko KONDO Ro OSAWA

出版者

BMFH Press

雑誌

Bioscience of Microbiota, Food and Health (ISSN:21863342)

巻号頁・発行日

vol.38, no.4, pp.159-163, 2019 (Released:2019-10-30)

参考文献数

31

被引用文献数

9

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Microbial production of butyrate is impaired in patients with ulcerative colitis (UC); however, this inhibition is not well understood in Japanese UC patients. Therefore, we quantitatively analyzed genes encoding butyryl-CoA:acetate CoA-transferase (but) and butyrate kinase (buk) in the gut microbiota of Japanese patients with UC and healthy volunteers (HVs). But showed higher levels than buk. Moreover, patients with UC showed significantly decreased levels of but associated with Roseburia sp./Eubacterium rectale compared with HVs. But, which is associated with Faecalibacterium sp., was maintained in patients with UC, with an unchanged relative abundance of Faecalibacterium sp. microorganisms in patients with UC compared with HVs.